DNA polymerase activity in encysting Entamoeba invadens

Asao Makioka, Masahiro Kumagai, Hiroshi Ohtomo, Seiki Kobayashi, Tsutomu Takeuchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using an axenic encystation system of Entamoeba invadens as a model for E. histolytica encystation, we examined the level of DNA polymerase activity in E. invadens during encystation induced in vitro. We first characterized the DNA polymerase activity of trophozoites of E. invadens, comparing it with that of E. histolytica, and found that the activity of E. invadens was lower than that of E. histolytica at pH 2, 4, and 6 and was higher at pH 8 and 10. The activity of E. invadens was completely inhibited by high concentrations of K+. Among inhibitors of mammalian DNA polymerases, aphidicolin and N- ethylmaleimide inhibited the activity, but 2',3'-dideoxythymidine-5'- triphosphate did not. Thus, the sensitivity of the E. invadens activity to salt and inhibitors of mammalian DNA polymerases was basically the same as that recorded for E. histolytica in our previous results. The level of DNA polymerase activity in cysts decreased as encystation proceeded as compared with that of trophozoites. The results indicate that encystation is accompanied by a reduced level of DNA polymerase activity, which correlates with the previous finding that nuclear division occurs during cyst maturation in the absence of DNA synthesis.

Original languageEnglish
Pages (from-to)604-606
Number of pages3
JournalParasitology Research
Volume85
Issue number7
DOIs
Publication statusPublished - 1999 Jul 1

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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    Makioka, A., Kumagai, M., Ohtomo, H., Kobayashi, S., & Takeuchi, T. (1999). DNA polymerase activity in encysting Entamoeba invadens. Parasitology Research, 85(7), 604-606. https://doi.org/10.1007/s004360050603