DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines

Hiroshi Nishihara, Masae Maeda, Atsushi Oda, Masumi Tsuda, Hirofumi Sawa, Kazuo Nagashima, Shinya Tanaka

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The CDM (ced-5 of Caenorhabditis elegans, DOCK180 [downstream of Crk with molecular weight of 180 kDa] of humans, and myoblast city of Drosophila melanogaster) family of proteins has been shown to play a pivotal role in the integrin-mediated signaling pathway under the regulation of an adaptor molecule c-CT10-related kinase II (c-Crk-II) in adherent cells. Recently, hematopoietic cell-specific CDM protein DOCK2 has been shown to be indispensable for lymphocyte migration. However, the regulatory mechanism for DOCK2 is still unknown because DOCK2 lacks a c-Crk-II binding consensus motif. In this study, we demonstrated that DOCK2 bound to CrkL, which is present exclusively in hematopoietic cells both in vivo and in vitro, and we also found that 2 separate regions of DOCK2 contributed to its binding to Src homology 3 (SH3) domain of CrkL. Colocalization of DOCK2 with Crk-like (CrkL) and F-actin was shown by immunocytochemical analysis with the use of Jurkat cells. We also found that CrkL-induced activation of small guanine triphosphatase (GTPase) Rac1 was significantly inhibited by the DOCK2-dCS mutant in 293T cells. Furthermore, the association of DOCK2 and Vav, the guanine-nucleotide exchanging factor (GEF) for Rac1, was demonstrated in Jurkat cells. Finally, the stable expression of DOCK2-dCS mutant in Jurkat cells was shown to reduce cell attachment. These data suggest the presence of a novel protein complex of CrkL, DOCK2, and Vav to regulate Rac1 in leukemia cell lines.

Original languageEnglish
Pages (from-to)3968-3974
Number of pages7
JournalBlood
Volume100
Issue number12
DOIs
Publication statusPublished - 2002 Dec 1
Externally publishedYes

Fingerprint

Jurkat Cells
Leukemia
Cells
Cell Line
Phosphotransferases
Proteins
Guanine Nucleotides
Lymphocytes
Guanine
Drosophila Proteins
Integrins
src Homology Domains
Actins
Myoblasts
HEK293 Cells
Caenorhabditis elegans
Chemical activation
Molecular weight
Association reactions
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Nishihara, H., Maeda, M., Oda, A., Tsuda, M., Sawa, H., Nagashima, K., & Tanaka, S. (2002). DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines. Blood, 100(12), 3968-3974. https://doi.org/10.1182/blood-2001-11-0032

DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines. / Nishihara, Hiroshi; Maeda, Masae; Oda, Atsushi; Tsuda, Masumi; Sawa, Hirofumi; Nagashima, Kazuo; Tanaka, Shinya.

In: Blood, Vol. 100, No. 12, 01.12.2002, p. 3968-3974.

Research output: Contribution to journalArticle

Nishihara, H, Maeda, M, Oda, A, Tsuda, M, Sawa, H, Nagashima, K & Tanaka, S 2002, 'DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines', Blood, vol. 100, no. 12, pp. 3968-3974. https://doi.org/10.1182/blood-2001-11-0032
Nishihara H, Maeda M, Oda A, Tsuda M, Sawa H, Nagashima K et al. DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines. Blood. 2002 Dec 1;100(12):3968-3974. https://doi.org/10.1182/blood-2001-11-0032
Nishihara, Hiroshi ; Maeda, Masae ; Oda, Atsushi ; Tsuda, Masumi ; Sawa, Hirofumi ; Nagashima, Kazuo ; Tanaka, Shinya. / DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines. In: Blood. 2002 ; Vol. 100, No. 12. pp. 3968-3974.
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