DOCK2 mediates T cell receptor-induced activation of Rac2 and IL-2 transcription

Hiroshi Nishihara, Masae Maeda, Masumi Tsuda, Yoshinori Makino, Hirofumi Sawa, Kazuo Nagashima, Shinya Tanaka

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

DOCK2, a CDM family protein exclusively found in hematopoietic cells, has been shown to play a role in lymphocyte migration by the regulation of actin cytoskeleton. Although DOCK2 has been shown to induce the activation of Rac1, the regulatory mechanism of Rac2, which is a hematopoietic cell-specific small GTPase, is still unknown. In this study, we examined the role of DOCK2 in the activation of Rac2 in hematopoietic cells. DOCK2 was found to associate with the ζ subunit of the CD3 complex of T cell receptors in Jurkat cells and to activate forced expressed Rac2 in 293T cells. In addition, the stable expression of DOCK2 in Jurkat cells exhibited the elevated activity of endogenous Rac2. Furthermore, the transcriptional activity of interleukin-2 (IL-2) was enhanced in DOCK2-expressing Jurkat cells and the dominant negative form of Rac2 suppressed its elevated IL-2 promoter activity. These results suggest that DOCK2 mediates TCR-dependent activation of Rac2, leading to the regulation of IL-2 promoter activity in T cells.

Original languageEnglish
Pages (from-to)716-720
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number3
DOIs
Publication statusPublished - 2002 Oct 14
Externally publishedYes

Keywords

  • DOCK2
  • Interleukin-2
  • Rac2
  • T cell antigen receptor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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