DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma

Lei Wang, Hiroshi Nishihara, Taichi Kimura, Yasutaka Kato, Mishie Tanino, Mitsufumi Nishio, Masato Obara, Tomoyuki Endo, Takao Koike, Shinya Tanaka

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18 Citations (Scopus)

Abstract

DOCK2; a member of the CDM protein family, regulates cell motility and cytokine production through the activation of Rac in mammalian hematopoietic cells and plays a pivotal role in the modulation of the immune system. Here we demonstrated the alternative function of DOCK2 in hematopoietic tumor cells, especially in terms of its association with the tumor progression. Immunostaining for DOCK2 in 20 cases of human B cell lymphoma tissue specimens including diffuse large B cell lymphoma and follicular lymphoma revealed the prominent expression of DOCK2 in all of the lymphoma cells. DOCK2-knockdown (KD) of the B cell lymphoma cell lines, Ramos and Raji, using the lentiviral shRNA system presented decreased cell proliferation compared to the control cells. Furthermore, the tumor formation of DOCK2-KD Ramos cell in nude mice was significantly abrogated. Western blotting analysis and pull-down assay using GST-PAK-RBD kimeric protein suggested the presence of DOCK2-Rac-ERK pathway regulating the cell proliferation of these lymphoma cells. This is the first report to clarify the prominent role of DOCK2 in hematopoietic malignancy.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume395
Issue number1
DOIs
Publication statusPublished - 2010 Apr
Externally publishedYes

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Keywords

  • B cell lymphoma
  • DOCK2
  • ERK
  • Hematopoietic malignancy
  • Rac

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Wang, L., Nishihara, H., Kimura, T., Kato, Y., Tanino, M., Nishio, M., Obara, M., Endo, T., Koike, T., & Tanaka, S. (2010). DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma. Biochemical and Biophysical Research Communications, 395(1), 111-115. https://doi.org/10.1016/j.bbrc.2010.03.148