Dopamine receptor activation reorganizes neuronal ensembles during hippocampal sharp waves in vitro

Takeyuki Miyawaki, Hiroaki Norimoto, Tomoe Ishikawa, Yusuke Watanabe, Norio Matsuki, Yuji Ikegaya

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hippocampal sharp wave (SW)/ripple complexes are thought to contribute to memory consolidation. Previous studies suggest that behavioral rewards facilitate SW occurrence in vivo. However, little is known about the precise mechanism underlying this enhancement. Here, we examined the effect of dopaminergic neuromodulation on spontaneously occurring SWs in acute hippocampal slices. Local field potentials were recorded from the CA1 region. A brief (1 min) treatment with dopamine led to a persistent increase in the event frequency and the magnitude of SWs. This effect lasted at least for our recording period of 45 min and did not occur in the presence of a dopamine D1/D 5 receptor antagonist. Functional multineuron calcium imaging revealed that dopamine-induced SW augmentation was associated with an enriched repertoire of the firing patterns in SW events, whereas the overall tendency of individual neurons to participate in SWs and the mean number of cells participating in a single SW were maintained. Therefore, dopaminergic activation is likely to reorganize cell assemblies during SWs.

Original languageEnglish
Article numbere104438
JournalPLoS One
Volume9
Issue number8
DOIs
Publication statusPublished - 2014 Aug 4
Externally publishedYes

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Dopamine Receptors
dopamine
Dopamine
Chemical activation
Reward
antagonists
Cell Count
neurons
image analysis
cells
Calcium
Neurons
calcium
receptors
Consolidation
In Vitro Techniques
dopamine receptors
Imaging techniques
Data storage equipment

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Dopamine receptor activation reorganizes neuronal ensembles during hippocampal sharp waves in vitro. / Miyawaki, Takeyuki; Norimoto, Hiroaki; Ishikawa, Tomoe; Watanabe, Yusuke; Matsuki, Norio; Ikegaya, Yuji.

In: PLoS One, Vol. 9, No. 8, e104438, 04.08.2014.

Research output: Contribution to journalArticle

Miyawaki, Takeyuki ; Norimoto, Hiroaki ; Ishikawa, Tomoe ; Watanabe, Yusuke ; Matsuki, Norio ; Ikegaya, Yuji. / Dopamine receptor activation reorganizes neuronal ensembles during hippocampal sharp waves in vitro. In: PLoS One. 2014 ; Vol. 9, No. 8.
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