Dose - Response of initial G2-chromatid breaks induced in normal human fibroblasts by heavy ions

T. Kawata, M. Durante, Y. Furusawa, K. George, N. Takai, H. Wu, F. A. Cucinotta

Research output: Contribution to journalArticle

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Abstract

Purpose: To investigate initial chromatid breaks in prematurely condensed G2 chromosomes following exposure to heavy ions of different LET. Material and methods: Exponentially growing human fibroblast cells AG 1522 were irradiated with γ-rays, energetic carbon (13 keV/μm, 80 keV/μm), silicon (55keV/μm) and iron (140 keV/μm, 185 keV/μm, 440 keV/μm) ions. Chromosomes were prematurely condensed using calyculin-A. Initial chromatid-type and isochromatid breaks in G2 cells were scored. Results: The dose-response curves for total chromatid breaks were linear regardless of radiation type. The relative biological effectiveness (RBE) showed a LET-dependent increase, peaking around 2.7 at 55-80 keV/μm and decreasing at higher LET. The dose - response curves for isochromatid-type breaks were linear for high-LET radiations, but linear-quadratic for γ-rays and 13 keV/μm carbon ions. The RBE for the induction of isochromatid breaks obtained from linear components increased rapidly between 13 keV/μm (about 7) and 80 keV/μm carbon (about 71), and decreased gradually until 440 keV/μm iron ions (about 66). Conclusions: High-LET radiations are more effective at inducing isochromatid breaks, while low-LET radiations are more effective at inducing chromatid-type breaks. The densely ionizing track structures of heavy ions and the proximity of sister chromatids in G2 cells result in an increase in isochromatid breaks.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalInternational Journal of Radiation Biology
Volume77
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Heavy Ions
Linear Energy Transfer
Chromatids
chromatids
fibroblasts
Fibroblasts
Heavy ions
dose response
heavy ions
ions
Radiation
dosage
chromosomes
radiation
Chromosomes
Relative Biological Effectiveness
Carbon
rays
Ions
carbon

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Dose - Response of initial G2-chromatid breaks induced in normal human fibroblasts by heavy ions. / Kawata, T.; Durante, M.; Furusawa, Y.; George, K.; Takai, N.; Wu, H.; Cucinotta, F. A.

In: International Journal of Radiation Biology, Vol. 77, No. 2, 2001, p. 165-174.

Research output: Contribution to journalArticle

Kawata, T, Durante, M, Furusawa, Y, George, K, Takai, N, Wu, H & Cucinotta, FA 2001, 'Dose - Response of initial G2-chromatid breaks induced in normal human fibroblasts by heavy ions', International Journal of Radiation Biology, vol. 77, no. 2, pp. 165-174. https://doi.org/10.1080/09553000010007686
Kawata, T. ; Durante, M. ; Furusawa, Y. ; George, K. ; Takai, N. ; Wu, H. ; Cucinotta, F. A. / Dose - Response of initial G2-chromatid breaks induced in normal human fibroblasts by heavy ions. In: International Journal of Radiation Biology. 2001 ; Vol. 77, No. 2. pp. 165-174.
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AU - Wu, H.

AU - Cucinotta, F. A.

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