Down-regulation of EGFR prolonged cell growth of glioma but did not increase the sensitivity to temozolomide

Nobuharu Inaba, Kouki Fujioka, Hidetsugu Saito, Masaki Kimura, Keiichi Ikeda, Yuriko Inoue, Sho Ishizawa, Yoshinobu Manome

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Malignant glioma is an invasive disease of the central nervous system. One of the factors that regulate growth of these tumors is expression of epidermal growth factor receptor (EGFR) in the cells. This study investigated the effects of down-regulation of EGFR on cell proliferation, cell cycle and cytotoxicity to antineoplastic agent. Materials and Methods: A short hairpin RNA transcription vector targeting EGFR was transfected into KNS42 cells. Growth curve, cell cycle and sensitivity to temozolomide of the cells were assessed. Results: Transfection inhibited EGFR expression by 50.5%. It prolonged cell doubling time by 25.7%. However, it did not meaningfully alter the cell cycle populations nor increase sensitivity to temozolomide. Conclusion: Suppressing expression of EGFR inhibited cell proliferation. However, unlike PTEN expression or ROCK1 down-regulation, it did not alter the cell cycle or increase sensitivity to temozolomide.

Original languageEnglish
Pages (from-to)3253-3257
Number of pages5
JournalAnticancer Research
Volume31
Issue number10
Publication statusPublished - 2011 Oct

Fingerprint

temozolomide
Epidermal Growth Factor Receptor
Glioma
Down-Regulation
Cell Cycle
Growth
Cell Proliferation
Central Nervous System Diseases
Antineoplastic Agents
Small Interfering RNA
Transfection
Intercellular Signaling Peptides and Proteins

Keywords

  • Cell cycle
  • EGFR
  • Glioma
  • Temozolomide

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Down-regulation of EGFR prolonged cell growth of glioma but did not increase the sensitivity to temozolomide. / Inaba, Nobuharu; Fujioka, Kouki; Saito, Hidetsugu; Kimura, Masaki; Ikeda, Keiichi; Inoue, Yuriko; Ishizawa, Sho; Manome, Yoshinobu.

In: Anticancer Research, Vol. 31, No. 10, 10.2011, p. 3253-3257.

Research output: Contribution to journalArticle

Inaba, N, Fujioka, K, Saito, H, Kimura, M, Ikeda, K, Inoue, Y, Ishizawa, S & Manome, Y 2011, 'Down-regulation of EGFR prolonged cell growth of glioma but did not increase the sensitivity to temozolomide', Anticancer Research, vol. 31, no. 10, pp. 3253-3257.
Inaba, Nobuharu ; Fujioka, Kouki ; Saito, Hidetsugu ; Kimura, Masaki ; Ikeda, Keiichi ; Inoue, Yuriko ; Ishizawa, Sho ; Manome, Yoshinobu. / Down-regulation of EGFR prolonged cell growth of glioma but did not increase the sensitivity to temozolomide. In: Anticancer Research. 2011 ; Vol. 31, No. 10. pp. 3253-3257.
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AU - Ikeda, Keiichi

AU - Inoue, Yuriko

AU - Ishizawa, Sho

AU - Manome, Yoshinobu

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AB - Background: Malignant glioma is an invasive disease of the central nervous system. One of the factors that regulate growth of these tumors is expression of epidermal growth factor receptor (EGFR) in the cells. This study investigated the effects of down-regulation of EGFR on cell proliferation, cell cycle and cytotoxicity to antineoplastic agent. Materials and Methods: A short hairpin RNA transcription vector targeting EGFR was transfected into KNS42 cells. Growth curve, cell cycle and sensitivity to temozolomide of the cells were assessed. Results: Transfection inhibited EGFR expression by 50.5%. It prolonged cell doubling time by 25.7%. However, it did not meaningfully alter the cell cycle populations nor increase sensitivity to temozolomide. Conclusion: Suppressing expression of EGFR inhibited cell proliferation. However, unlike PTEN expression or ROCK1 down-regulation, it did not alter the cell cycle or increase sensitivity to temozolomide.

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