Downmodulation of IL-8 receptors, type A and type B, on human lung neutrophils in vivo

Kenzo Soejima, Seitaro Fujishima, Hidetoshi Nakamura, Yasuhiro Waki, Morio Nakamura, Hiroaki Matsubara, Sadatomo Tasaka, Koichi Sayama, Akitoshi Ishizaka, Minoru Kanazawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We examined the expression of interleukin (IL)-8 receptors (Rs), type A (IL-8-RA) and type B (IL-8-RB), on peripheral blood and bronchoalveolar lavage (BAL) fluid neutrophils; we also examined IL-8 and other chemoattractants in the epithelial lining fluid (ELF) of patients with chronic lower respiratory tract infection (CLI) to elucidate the in vivo regulation of IL-8Rs. Neutrophils were stained with monoclonal antibodies specific for IL-8-RA and IL-8-RB. We detected higher levels of IL-8 (81.6 ± 25.4 ng/ml, mean ± SE), leukotriene (LT) B4, and IL-1β in the ELF of the CLI patients than in their serum (P < 0.05). The expression of IL-8Rs on BAL neutrophils was significantly lower than that on peripheral blood neutrophils (P < 0.01 for both). In vitro analysis showed that low-level IL-8 (50 ng/ml) alone did not affect IL-8R expression but that it was downregulated by high- level IL-8 (500 ng/ml) alone and by low-level IL-8 in combination with LTB4 or IL-1β. Staurosporine reduced the downmodulation by low-level IL-8 plus LTB4 or IL-1β but not by high-level IL-8 alone. We speculate that pulmonary IL-8-RA and IL-8-RB may have been downmodulated by the combined effect of local chemoattractants through, in part, a protein kinase C-dependent mechanism.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume273
Issue number3 17-3
Publication statusPublished - 1997 Sep

Fingerprint

Interleukin-8A Receptors
Interleukin-8B Receptors
Interleukin-8
Neutrophils
Lung
Leukotriene B4
Interleukins
Interleukin-1
Chemotactic Factors
Staurosporine
Bronchoalveolar Lavage Fluid
Bronchoalveolar Lavage

Keywords

  • Bronchiectasis
  • Bronchoalveolar lavage
  • Interleukin- 1
  • Interleukin-8 receptors
  • Leukotriene B

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Downmodulation of IL-8 receptors, type A and type B, on human lung neutrophils in vivo. / Soejima, Kenzo; Fujishima, Seitaro; Nakamura, Hidetoshi; Waki, Yasuhiro; Nakamura, Morio; Matsubara, Hiroaki; Tasaka, Sadatomo; Sayama, Koichi; Ishizaka, Akitoshi; Kanazawa, Minoru.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 273, No. 3 17-3, 09.1997.

Research output: Contribution to journalArticle

Soejima, K, Fujishima, S, Nakamura, H, Waki, Y, Nakamura, M, Matsubara, H, Tasaka, S, Sayama, K, Ishizaka, A & Kanazawa, M 1997, 'Downmodulation of IL-8 receptors, type A and type B, on human lung neutrophils in vivo', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 273, no. 3 17-3.
Soejima, Kenzo ; Fujishima, Seitaro ; Nakamura, Hidetoshi ; Waki, Yasuhiro ; Nakamura, Morio ; Matsubara, Hiroaki ; Tasaka, Sadatomo ; Sayama, Koichi ; Ishizaka, Akitoshi ; Kanazawa, Minoru. / Downmodulation of IL-8 receptors, type A and type B, on human lung neutrophils in vivo. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1997 ; Vol. 273, No. 3 17-3.
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AU - Fujishima, Seitaro

AU - Nakamura, Hidetoshi

AU - Waki, Yasuhiro

AU - Nakamura, Morio

AU - Matsubara, Hiroaki

AU - Tasaka, Sadatomo

AU - Sayama, Koichi

AU - Ishizaka, Akitoshi

AU - Kanazawa, Minoru

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N2 - We examined the expression of interleukin (IL)-8 receptors (Rs), type A (IL-8-RA) and type B (IL-8-RB), on peripheral blood and bronchoalveolar lavage (BAL) fluid neutrophils; we also examined IL-8 and other chemoattractants in the epithelial lining fluid (ELF) of patients with chronic lower respiratory tract infection (CLI) to elucidate the in vivo regulation of IL-8Rs. Neutrophils were stained with monoclonal antibodies specific for IL-8-RA and IL-8-RB. We detected higher levels of IL-8 (81.6 ± 25.4 ng/ml, mean ± SE), leukotriene (LT) B4, and IL-1β in the ELF of the CLI patients than in their serum (P < 0.05). The expression of IL-8Rs on BAL neutrophils was significantly lower than that on peripheral blood neutrophils (P < 0.01 for both). In vitro analysis showed that low-level IL-8 (50 ng/ml) alone did not affect IL-8R expression but that it was downregulated by high- level IL-8 (500 ng/ml) alone and by low-level IL-8 in combination with LTB4 or IL-1β. Staurosporine reduced the downmodulation by low-level IL-8 plus LTB4 or IL-1β but not by high-level IL-8 alone. We speculate that pulmonary IL-8-RA and IL-8-RB may have been downmodulated by the combined effect of local chemoattractants through, in part, a protein kinase C-dependent mechanism.

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