Downregulation of functional Reelin receptors in projection neurons implies that primary Reelin action occurs at early/premigratory stages

Takayuki Uchida, Atsushi Baba, F. Javier Pérez-Martínez, Terumasa Hibi, Takaki Miyata, Juan M. Luque, Kazunori Nakajima, Mitsuharu Hattori

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Reelin signaling is essential for correct development of the mammalian brain. Reelin binds to apolipoprotein E receptor 2 and very low-density lipoprotein receptor and induces phosphorylation of Dab1. However, when and where these reactions occur is essentially unknown, and the primary function(s) of Reelin remain unclear. Here, we used alkaline phosphatase fusion of the receptor-binding region of Reelin to quantitatively investigate the localization of functional Reelin receptors (i.e., those on the plasma membrane as mature forms) in the developing brain. In the wild-type cerebral cortex, they are mainly present in the intermediate and subventricular zones, as well as in radial fibers, but much less in the cell bodies of the cortical plate. Functional Reelin receptors are much more abundant in the Reelin-deficient cortical plate, indicating that Reelin induces their downregulation and that it begins before the neurons migrate out of the intermediate zone. In the wild-type cerebellum, functional Reelin receptors are mainly present in the cerebellar ventricular zone but scarcely expressed by Purkinje cells that have migrated out of it. It is thus strongly suggested that Reelin exerts critical actions on migrating projection neurons at their early/premigratory stages en route to their final destinations, in the developing cerebral cortex and cerebellum.

Original languageEnglish
Pages (from-to)10653-10662
Number of pages10
JournalJournal of Neuroscience
Volume29
Issue number34
DOIs
Publication statusPublished - 2009 Aug 26

Fingerprint

Cerebral Cortex
Down-Regulation
Neurons
Cerebellum
Lateral Ventricles
Purkinje Cells
Brain
Alkaline Phosphatase
Phosphorylation
Cell Membrane
reelin receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Downregulation of functional Reelin receptors in projection neurons implies that primary Reelin action occurs at early/premigratory stages. / Uchida, Takayuki; Baba, Atsushi; Pérez-Martínez, F. Javier; Hibi, Terumasa; Miyata, Takaki; Luque, Juan M.; Nakajima, Kazunori; Hattori, Mitsuharu.

In: Journal of Neuroscience, Vol. 29, No. 34, 26.08.2009, p. 10653-10662.

Research output: Contribution to journalArticle

Uchida, Takayuki ; Baba, Atsushi ; Pérez-Martínez, F. Javier ; Hibi, Terumasa ; Miyata, Takaki ; Luque, Juan M. ; Nakajima, Kazunori ; Hattori, Mitsuharu. / Downregulation of functional Reelin receptors in projection neurons implies that primary Reelin action occurs at early/premigratory stages. In: Journal of Neuroscience. 2009 ; Vol. 29, No. 34. pp. 10653-10662.
@article{37152a92f6b14c6eb059a059f71c5351,
title = "Downregulation of functional Reelin receptors in projection neurons implies that primary Reelin action occurs at early/premigratory stages",
abstract = "Reelin signaling is essential for correct development of the mammalian brain. Reelin binds to apolipoprotein E receptor 2 and very low-density lipoprotein receptor and induces phosphorylation of Dab1. However, when and where these reactions occur is essentially unknown, and the primary function(s) of Reelin remain unclear. Here, we used alkaline phosphatase fusion of the receptor-binding region of Reelin to quantitatively investigate the localization of functional Reelin receptors (i.e., those on the plasma membrane as mature forms) in the developing brain. In the wild-type cerebral cortex, they are mainly present in the intermediate and subventricular zones, as well as in radial fibers, but much less in the cell bodies of the cortical plate. Functional Reelin receptors are much more abundant in the Reelin-deficient cortical plate, indicating that Reelin induces their downregulation and that it begins before the neurons migrate out of the intermediate zone. In the wild-type cerebellum, functional Reelin receptors are mainly present in the cerebellar ventricular zone but scarcely expressed by Purkinje cells that have migrated out of it. It is thus strongly suggested that Reelin exerts critical actions on migrating projection neurons at their early/premigratory stages en route to their final destinations, in the developing cerebral cortex and cerebellum.",
author = "Takayuki Uchida and Atsushi Baba and P{\'e}rez-Mart{\'i}nez, {F. Javier} and Terumasa Hibi and Takaki Miyata and Luque, {Juan M.} and Kazunori Nakajima and Mitsuharu Hattori",
year = "2009",
month = "8",
day = "26",
doi = "10.1523/JNEUROSCI.0345-09.2009",
language = "English",
volume = "29",
pages = "10653--10662",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "34",

}

TY - JOUR

T1 - Downregulation of functional Reelin receptors in projection neurons implies that primary Reelin action occurs at early/premigratory stages

AU - Uchida, Takayuki

AU - Baba, Atsushi

AU - Pérez-Martínez, F. Javier

AU - Hibi, Terumasa

AU - Miyata, Takaki

AU - Luque, Juan M.

AU - Nakajima, Kazunori

AU - Hattori, Mitsuharu

PY - 2009/8/26

Y1 - 2009/8/26

N2 - Reelin signaling is essential for correct development of the mammalian brain. Reelin binds to apolipoprotein E receptor 2 and very low-density lipoprotein receptor and induces phosphorylation of Dab1. However, when and where these reactions occur is essentially unknown, and the primary function(s) of Reelin remain unclear. Here, we used alkaline phosphatase fusion of the receptor-binding region of Reelin to quantitatively investigate the localization of functional Reelin receptors (i.e., those on the plasma membrane as mature forms) in the developing brain. In the wild-type cerebral cortex, they are mainly present in the intermediate and subventricular zones, as well as in radial fibers, but much less in the cell bodies of the cortical plate. Functional Reelin receptors are much more abundant in the Reelin-deficient cortical plate, indicating that Reelin induces their downregulation and that it begins before the neurons migrate out of the intermediate zone. In the wild-type cerebellum, functional Reelin receptors are mainly present in the cerebellar ventricular zone but scarcely expressed by Purkinje cells that have migrated out of it. It is thus strongly suggested that Reelin exerts critical actions on migrating projection neurons at their early/premigratory stages en route to their final destinations, in the developing cerebral cortex and cerebellum.

AB - Reelin signaling is essential for correct development of the mammalian brain. Reelin binds to apolipoprotein E receptor 2 and very low-density lipoprotein receptor and induces phosphorylation of Dab1. However, when and where these reactions occur is essentially unknown, and the primary function(s) of Reelin remain unclear. Here, we used alkaline phosphatase fusion of the receptor-binding region of Reelin to quantitatively investigate the localization of functional Reelin receptors (i.e., those on the plasma membrane as mature forms) in the developing brain. In the wild-type cerebral cortex, they are mainly present in the intermediate and subventricular zones, as well as in radial fibers, but much less in the cell bodies of the cortical plate. Functional Reelin receptors are much more abundant in the Reelin-deficient cortical plate, indicating that Reelin induces their downregulation and that it begins before the neurons migrate out of the intermediate zone. In the wild-type cerebellum, functional Reelin receptors are mainly present in the cerebellar ventricular zone but scarcely expressed by Purkinje cells that have migrated out of it. It is thus strongly suggested that Reelin exerts critical actions on migrating projection neurons at their early/premigratory stages en route to their final destinations, in the developing cerebral cortex and cerebellum.

UR - http://www.scopus.com/inward/record.url?scp=69449088849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69449088849&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.0345-09.2009

DO - 10.1523/JNEUROSCI.0345-09.2009

M3 - Article

VL - 29

SP - 10653

EP - 10662

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 34

ER -