Downregulation of Notch mediates the seamless transition of individual Drosophila neuroepithelial progenitors into optic medullar neuroblasts during prolonged G1

Minako Orihara-Ono, Masako Toriya, Keiko Nakao, Hideyuki Okano

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The first step in the development of the Drosophila optic medullar primordia is the expansion of symmetrically dividing neuroepithelial cells (NEs); this step is then followed by the appearance of asymmetrically dividing neuroblasts (NBs). However, the mechanisms responsible for the change from NEs to NBs remain unclear. Here, we performed detailed analyses demonstrating that individual NEs are converted into NBs. We also showed that this transition occurs during an elongated G1 phase. During this G1 phase, the morphological features and gene expressions of each columnar NE changed dynamically. Once the NE-to-NB transition was completed, the former NE changed its cell-cycling behavior, commencing asymmetric division. We also found that Notch signaling pathway was activated just before the transition and was rapidly downregulated. Furthermore, the clonal loss of the Notch wild copy in the NE region near the medial edge caused the ectopic accumulation of Delta, leading to the precocious onset of transition. Taken together, these findings indicate that the activation of Notch signaling during a finite window coordinates the proper timing of the NE-to-NB transition.

Original languageEnglish
Pages (from-to)163-175
Number of pages13
JournalDevelopmental Biology
Volume351
Issue number1
DOIs
Publication statusPublished - 2011 Mar 1

Keywords

  • Drosophila
  • Neuroblast
  • Neuroepithelium
  • Notch
  • Optic lobe

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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