Dpysl4 is involved in tooth germ morphogenesis through growth regulation, polarization and differentiation of dental epithelial cells

Masato Yasukawa, Kentaro Ishida, Yohei Yuge, Mai Hanaoka, Yoko Minami, Miho Ogawa, Takashi Sasaki, Masahiro Saito, Takashi Tsuji

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Dihydropyrimidinase-related protein 4 (Dpysl4) is a known regulator of hippocampal neuron development. Here, we report that Dpysl4 is involved in growth regulation, polarization and differentiation of dental epithelial cells during tooth germ morphogenesis. A reduction in Dpysl4 gene expression in the tooth germ produced a loss of ameloblasts, resulting in the decrease of synthesis and secretion of enamel. The inhibition of Dpysl4 gene expression led to promotion of cell proliferation of inner enamel epithelial cells and inhibition of the differentiation of these cells into pre-ameloblasts, which was confirmed by analyzing cell polarization, columnar cell structure formation and the expression of ameloblast marker genes. By contrast, overexpression of Dpysl4 in dental epithelial cells induces inhibition of growth and increases the expression of the inner enamel epithelial cell marker gene, Msx2. These findings suggest that Dpysl4 plays essential roles in tooth germ morphogenesis through the regulation of dental epithelial cell proliferation, cell polarization and differentiation.

Original languageEnglish
Pages (from-to)382-390
Number of pages9
JournalInternational Journal of Biological Sciences
Volume9
Issue number4
DOIs
Publication statusPublished - 2013 Apr 26
Externally publishedYes

Fingerprint

Tooth Germ
enamel
morphogenesis
Morphogenesis
tooth
Ameloblasts
Tooth
teeth
epithelial cells
polarization
Epithelial Cells
tooth enamel
Dental Enamel
gene expression
Growth
dihydropyrimidinase
gene
Cell Differentiation
secretion
Cell Proliferation

Keywords

  • Cell polarization
  • Development
  • Differentiation
  • Dpysl4
  • Tooth germ

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Cite this

Dpysl4 is involved in tooth germ morphogenesis through growth regulation, polarization and differentiation of dental epithelial cells. / Yasukawa, Masato; Ishida, Kentaro; Yuge, Yohei; Hanaoka, Mai; Minami, Yoko; Ogawa, Miho; Sasaki, Takashi; Saito, Masahiro; Tsuji, Takashi.

In: International Journal of Biological Sciences, Vol. 9, No. 4, 26.04.2013, p. 382-390.

Research output: Contribution to journalArticle

Yasukawa, Masato ; Ishida, Kentaro ; Yuge, Yohei ; Hanaoka, Mai ; Minami, Yoko ; Ogawa, Miho ; Sasaki, Takashi ; Saito, Masahiro ; Tsuji, Takashi. / Dpysl4 is involved in tooth germ morphogenesis through growth regulation, polarization and differentiation of dental epithelial cells. In: International Journal of Biological Sciences. 2013 ; Vol. 9, No. 4. pp. 382-390.
@article{c4fc07e358d3494986746ce0b451866b,
title = "Dpysl4 is involved in tooth germ morphogenesis through growth regulation, polarization and differentiation of dental epithelial cells",
abstract = "Dihydropyrimidinase-related protein 4 (Dpysl4) is a known regulator of hippocampal neuron development. Here, we report that Dpysl4 is involved in growth regulation, polarization and differentiation of dental epithelial cells during tooth germ morphogenesis. A reduction in Dpysl4 gene expression in the tooth germ produced a loss of ameloblasts, resulting in the decrease of synthesis and secretion of enamel. The inhibition of Dpysl4 gene expression led to promotion of cell proliferation of inner enamel epithelial cells and inhibition of the differentiation of these cells into pre-ameloblasts, which was confirmed by analyzing cell polarization, columnar cell structure formation and the expression of ameloblast marker genes. By contrast, overexpression of Dpysl4 in dental epithelial cells induces inhibition of growth and increases the expression of the inner enamel epithelial cell marker gene, Msx2. These findings suggest that Dpysl4 plays essential roles in tooth germ morphogenesis through the regulation of dental epithelial cell proliferation, cell polarization and differentiation.",
keywords = "Cell polarization, Development, Differentiation, Dpysl4, Tooth germ",
author = "Masato Yasukawa and Kentaro Ishida and Yohei Yuge and Mai Hanaoka and Yoko Minami and Miho Ogawa and Takashi Sasaki and Masahiro Saito and Takashi Tsuji",
year = "2013",
month = "4",
day = "26",
doi = "10.7150/ijbs.5510",
language = "English",
volume = "9",
pages = "382--390",
journal = "International Journal of Biological Sciences",
issn = "1449-2288",
publisher = "Ivyspring International Publisher",
number = "4",

}

TY - JOUR

T1 - Dpysl4 is involved in tooth germ morphogenesis through growth regulation, polarization and differentiation of dental epithelial cells

AU - Yasukawa, Masato

AU - Ishida, Kentaro

AU - Yuge, Yohei

AU - Hanaoka, Mai

AU - Minami, Yoko

AU - Ogawa, Miho

AU - Sasaki, Takashi

AU - Saito, Masahiro

AU - Tsuji, Takashi

PY - 2013/4/26

Y1 - 2013/4/26

N2 - Dihydropyrimidinase-related protein 4 (Dpysl4) is a known regulator of hippocampal neuron development. Here, we report that Dpysl4 is involved in growth regulation, polarization and differentiation of dental epithelial cells during tooth germ morphogenesis. A reduction in Dpysl4 gene expression in the tooth germ produced a loss of ameloblasts, resulting in the decrease of synthesis and secretion of enamel. The inhibition of Dpysl4 gene expression led to promotion of cell proliferation of inner enamel epithelial cells and inhibition of the differentiation of these cells into pre-ameloblasts, which was confirmed by analyzing cell polarization, columnar cell structure formation and the expression of ameloblast marker genes. By contrast, overexpression of Dpysl4 in dental epithelial cells induces inhibition of growth and increases the expression of the inner enamel epithelial cell marker gene, Msx2. These findings suggest that Dpysl4 plays essential roles in tooth germ morphogenesis through the regulation of dental epithelial cell proliferation, cell polarization and differentiation.

AB - Dihydropyrimidinase-related protein 4 (Dpysl4) is a known regulator of hippocampal neuron development. Here, we report that Dpysl4 is involved in growth regulation, polarization and differentiation of dental epithelial cells during tooth germ morphogenesis. A reduction in Dpysl4 gene expression in the tooth germ produced a loss of ameloblasts, resulting in the decrease of synthesis and secretion of enamel. The inhibition of Dpysl4 gene expression led to promotion of cell proliferation of inner enamel epithelial cells and inhibition of the differentiation of these cells into pre-ameloblasts, which was confirmed by analyzing cell polarization, columnar cell structure formation and the expression of ameloblast marker genes. By contrast, overexpression of Dpysl4 in dental epithelial cells induces inhibition of growth and increases the expression of the inner enamel epithelial cell marker gene, Msx2. These findings suggest that Dpysl4 plays essential roles in tooth germ morphogenesis through the regulation of dental epithelial cell proliferation, cell polarization and differentiation.

KW - Cell polarization

KW - Development

KW - Differentiation

KW - Dpysl4

KW - Tooth germ

UR - http://www.scopus.com/inward/record.url?scp=84876993179&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876993179&partnerID=8YFLogxK

U2 - 10.7150/ijbs.5510

DO - 10.7150/ijbs.5510

M3 - Article

C2 - 23630450

AN - SCOPUS:84876993179

VL - 9

SP - 382

EP - 390

JO - International Journal of Biological Sciences

JF - International Journal of Biological Sciences

SN - 1449-2288

IS - 4

ER -