Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

Yusuke Miyauchi, Robert Kourist, Daisuke Uemura, Kenji Miyamoto

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

Original languageEnglish
Pages (from-to)7503-7505
Number of pages3
JournalChemical Communications
Volume47
Issue number26
DOIs
Publication statusPublished - 2011 Jul 14

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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