Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex

Seika Inoue, Kanehiro Hayashi, Kyota Fujita, Kazuhiko Tagawa, Hitoshi Okazawa, Kenichiro Kubo, Kazunori Nakajima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The actin cytoskeleton is crucial for neuronal migration in the mammalian developing cerebral cortex. The adaptor protein Drebrin-like (Dbnl) plays important roles in reorganization of the actin cytoskeleton, dendrite formation, and endocytosis by interacting with F-actin, cobl, and dynamin. Although Dbnl is known to be expressed in the brain, the functions of this molecule during brain development are largely unknown. In this study, to examine the roles of Dbnl in the developing cerebral cortex, we conducted experiments using mice of both sexes with knockdown of Dbnl, effected by in utero electroporation, in the migrating neurons of the embryonic cortex. Time-lapse imaging of the Dbnl-knockdown neurons revealed that the presence of Dbnl is a prerequisite for appropriate formation of processes in the multipolar neurons in the multipolar cell accumulation zone or the deep part of the subventricular zone, and for neuronal polarization and entry into the cortical plate. We found that Dbnl knockdown decreased the amount of N-cadherin protein expressed on the plasma membrane of the cortical neurons. The defect in neuronal migration caused by Dbnl knockdown was rescued by moderate overexpression of N-cadherin and αN-catenin or by transfection of the phospho-mimic form(Y337E, Y347E), but not the phospho-resistant form(Y337F, Y347F), of Dbnl. These results suggest that Dbnl controls neuronal migration, neuronal multipolar morphology, and cell polarity in the developing cerebral cortex via regulating N-cadherin expression.

Original languageEnglish
Pages (from-to)678-691
Number of pages14
JournalJournal of Neuroscience
Volume39
Issue number4
DOIs
Publication statusPublished - 2019 Jan 23

Fingerprint

Cadherins
Cerebral Cortex
Neurons
Actin Cytoskeleton
Time-Lapse Imaging
drebrins
Dynamins
Catenins
Cell Polarity
Electroporation
Lateral Ventricles
Brain
Dendrites
Endocytosis
Transfection
Actins
Proteins
Cell Membrane

Keywords

  • Cerebral cortex
  • Development
  • Neuronal migration
  • Phosphorylation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex. / Inoue, Seika; Hayashi, Kanehiro; Fujita, Kyota; Tagawa, Kazuhiko; Okazawa, Hitoshi; Kubo, Kenichiro; Nakajima, Kazunori.

In: Journal of Neuroscience, Vol. 39, No. 4, 23.01.2019, p. 678-691.

Research output: Contribution to journalArticle

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