TY - GEN
T1 - Droplet network connected by biological nanopores for DNA computing
AU - Yasuga, H.
AU - Kawano, R.
AU - Takinoue, M.
AU - Tsuji, Y.
AU - Osaki, T.
AU - Kamiya, K.
AU - Miki, N.
AU - Takeuchi, S.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - This paper experimentally describes formation of lipid-coated droplet network and demonstrates a biological NAND logic gate using this network and DNA. Droplets which compose this network are comparted by bilayer lipid membranes (BLMs) and connected via biological nanopores of membrane proteins, which ssDNA passes through. In the geometry of the droplet network, four droplets were placed at the corners and the center of an equilateral triangle. Inputs corresponded to the DNA translocation via biological nanopores from the two droplets at the corners (#1 and 2 in Fig. 1) to the center (#3 in Fig. 1), which can be controlled by the applied electrical fields between the droplets. Output was determined by whether DNA translocation occurred through nanopores between the center (#3 in Fig. 1) and the other corner droplet (#4 in Fig. 1). We believe that this system enables direct connection between electrical systems and computing with biomolecules.
AB - This paper experimentally describes formation of lipid-coated droplet network and demonstrates a biological NAND logic gate using this network and DNA. Droplets which compose this network are comparted by bilayer lipid membranes (BLMs) and connected via biological nanopores of membrane proteins, which ssDNA passes through. In the geometry of the droplet network, four droplets were placed at the corners and the center of an equilateral triangle. Inputs corresponded to the DNA translocation via biological nanopores from the two droplets at the corners (#1 and 2 in Fig. 1) to the center (#3 in Fig. 1), which can be controlled by the applied electrical fields between the droplets. Output was determined by whether DNA translocation occurred through nanopores between the center (#3 in Fig. 1) and the other corner droplet (#4 in Fig. 1). We believe that this system enables direct connection between electrical systems and computing with biomolecules.
KW - Bilayer Lipid Membrane
KW - Biological Nanopore
KW - DNA
KW - Droplet Contact Method
KW - Droplet Network
KW - Streptolysin O
KW - α-Hemolysin
UR - http://www.scopus.com/inward/record.url?scp=84891695675&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891695675&partnerID=8YFLogxK
U2 - 10.1109/Transducers.2013.6626994
DO - 10.1109/Transducers.2013.6626994
M3 - Conference contribution
AN - SCOPUS:84891695675
SN - 9781467359818
T3 - 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
SP - 1221
EP - 1222
BT - 2013 Transducers and Eurosensors XXVII
T2 - 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Y2 - 16 June 2013 through 20 June 2013
ER -