Droplet split-and-contact method for high-throughput transmembrane electrical recording

Yutaro Tsuji, Ryuji Kawano, Toshihisa Osaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

This paper describes the rapid and repetitive formation of planar lipid bilayers via a mechanical droplet contact method for high-throughput ion channel analysis. In this method, first, an aqueous droplet delivered in a lipid-in-oil solution is mechanically divided into two small droplets. Second, the two small droplets contact each other, resulting in the lipid bilayer formation. Third, an ion channel is immediately reconstituted into the bilayer and the transmembrane current signals are measured. By repeating this procedure, massive data sets of the channel signals can be obtained. This method allowed us to perform statistical analysis of α-hemolysin conductance (n = 256 within 30 min) and channel inhibition experiments by contacting different types of the droplets in a short time frame.

Original languageEnglish
Pages (from-to)10913-10919
Number of pages7
JournalAnalytical Chemistry
Volume85
Issue number22
DOIs
Publication statusPublished - 2013 Nov 19

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Contacts (fluid mechanics)
Throughput
Lipid bilayers
Ion Channels
Hemolysin Proteins
Statistical methods
Oils
Lipids
Experiments

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Droplet split-and-contact method for high-throughput transmembrane electrical recording. / Tsuji, Yutaro; Kawano, Ryuji; Osaki, Toshihisa; Kamiya, Koki; Miki, Norihisa; Takeuchi, Shoji.

In: Analytical Chemistry, Vol. 85, No. 22, 19.11.2013, p. 10913-10919.

Research output: Contribution to journalArticle

Tsuji, Y, Kawano, R, Osaki, T, Kamiya, K, Miki, N & Takeuchi, S 2013, 'Droplet split-and-contact method for high-throughput transmembrane electrical recording', Analytical Chemistry, vol. 85, no. 22, pp. 10913-10919. https://doi.org/10.1021/ac402299z
Tsuji, Yutaro ; Kawano, Ryuji ; Osaki, Toshihisa ; Kamiya, Koki ; Miki, Norihisa ; Takeuchi, Shoji. / Droplet split-and-contact method for high-throughput transmembrane electrical recording. In: Analytical Chemistry. 2013 ; Vol. 85, No. 22. pp. 10913-10919.
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