Drosophila big brain does not act as a water channel, but mediates cell adhesion

Kimiko Tatsumi, Shoji Tsuji, Hideki Miwa, Toshinori Morisaku, Mutsuo Nuriya, Minako Orihara, Kazunari Kaneko, Hideyuki Okano, Masato Yasui

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.

Original languageEnglish
Pages (from-to)2077-2082
Number of pages6
JournalFEBS Letters
Volume583
Issue number12
DOIs
Publication statusPublished - 2009 Jun 18

Fingerprint

Aquaporins
Cell adhesion
Cell Adhesion
Drosophila
Brain
Adhesives
Aquaporin 4
Ions
Water
Permeation
Neurogenesis
Assays
Suspensions
Agglomeration
Genes
Permeability
Cell Aggregation
Sequence Homology

Keywords

  • Aquaporin-4 (AQP4)
  • Big brain
  • Cell adhesion
  • Drosophila

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Drosophila big brain does not act as a water channel, but mediates cell adhesion. / Tatsumi, Kimiko; Tsuji, Shoji; Miwa, Hideki; Morisaku, Toshinori; Nuriya, Mutsuo; Orihara, Minako; Kaneko, Kazunari; Okano, Hideyuki; Yasui, Masato.

In: FEBS Letters, Vol. 583, No. 12, 18.06.2009, p. 2077-2082.

Research output: Contribution to journalArticle

Tatsumi, Kimiko ; Tsuji, Shoji ; Miwa, Hideki ; Morisaku, Toshinori ; Nuriya, Mutsuo ; Orihara, Minako ; Kaneko, Kazunari ; Okano, Hideyuki ; Yasui, Masato. / Drosophila big brain does not act as a water channel, but mediates cell adhesion. In: FEBS Letters. 2009 ; Vol. 583, No. 12. pp. 2077-2082.
@article{4ad6785284aa41549553663a67f8e084,
title = "Drosophila big brain does not act as a water channel, but mediates cell adhesion",
abstract = "The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.",
keywords = "Aquaporin-4 (AQP4), Big brain, Cell adhesion, Drosophila",
author = "Kimiko Tatsumi and Shoji Tsuji and Hideki Miwa and Toshinori Morisaku and Mutsuo Nuriya and Minako Orihara and Kazunari Kaneko and Hideyuki Okano and Masato Yasui",
year = "2009",
month = "6",
day = "18",
doi = "10.1016/j.febslet.2009.05.035",
language = "English",
volume = "583",
pages = "2077--2082",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "12",

}

TY - JOUR

T1 - Drosophila big brain does not act as a water channel, but mediates cell adhesion

AU - Tatsumi, Kimiko

AU - Tsuji, Shoji

AU - Miwa, Hideki

AU - Morisaku, Toshinori

AU - Nuriya, Mutsuo

AU - Orihara, Minako

AU - Kaneko, Kazunari

AU - Okano, Hideyuki

AU - Yasui, Masato

PY - 2009/6/18

Y1 - 2009/6/18

N2 - The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.

AB - The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.

KW - Aquaporin-4 (AQP4)

KW - Big brain

KW - Cell adhesion

KW - Drosophila

UR - http://www.scopus.com/inward/record.url?scp=67349176604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349176604&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2009.05.035

DO - 10.1016/j.febslet.2009.05.035

M3 - Article

C2 - 19467350

AN - SCOPUS:67349176604

VL - 583

SP - 2077

EP - 2082

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 12

ER -