Drosophila big brain does not act as a water channel, but mediates cell adhesion

Kimiko Tatsumi, Shoji Tsuji, Hideki Miwa, Toshinori Morisaku, Mutsuo Nuriya, Minako Orihara, Kazunari Kaneko, Hideyuki Okano, Masato Yasui

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.

Original languageEnglish
Pages (from-to)2077-2082
Number of pages6
JournalFEBS Letters
Volume583
Issue number12
DOIs
Publication statusPublished - 2009 Jun 18

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Keywords

  • Aquaporin-4 (AQP4)
  • Big brain
  • Cell adhesion
  • Drosophila

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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