Drosophila caspase transduces Shaggy/GSK-3β kinase activity in neural precursor development

Hirotaka Kanuka, Erina Kuranaga, Kiwamu Takemoto, Tetsuo Hiratou, Hideyuki Okano, Masayuki Miura

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Caspases are well known for their role in the execution of apoptotic programs, in which they cleave specific target proteins, leading to the elimination of cells, and for their role in cytokine maturation. In this study, we identified a novel substrate, which, through cleavage by caspases, can regulate Drosophila neural precursor development. Shaggy (Sgg)46 protein, an isoform encoded by the sgg gene and essential for the negative regulation of Wingless signaling, is cleaved by the Dark-dependent caspase. This cleavage converts it to an active kinase, which contributes to the formation of neural precursor (sensory organ precursor (SOP)) cells. Our evidence suggests that caspase regulation of the wingless pathway is not associated with apoptotic cell death. These results imply a novel role for caspases in modulating cell signaling pathways through substrate cleavage in neural precursor development.

Original languageEnglish
Pages (from-to)3793-3806
Number of pages14
JournalEMBO Journal
Volume24
Issue number21
DOIs
Publication statusPublished - 2005 Nov 2

Fingerprint

Glycogen Synthase Kinase 3
Caspases
Drosophila
Phosphotransferases
Cell signaling
Essential Genes
Cell death
Substrates
Protein Isoforms
Cell Death
Genes
Cytokines
Proteins

Keywords

  • Apoptosis
  • Caspase
  • Cell death
  • Drosophila
  • Kinase

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Drosophila caspase transduces Shaggy/GSK-3β kinase activity in neural precursor development. / Kanuka, Hirotaka; Kuranaga, Erina; Takemoto, Kiwamu; Hiratou, Tetsuo; Okano, Hideyuki; Miura, Masayuki.

In: EMBO Journal, Vol. 24, No. 21, 02.11.2005, p. 3793-3806.

Research output: Contribution to journalArticle

Kanuka, H, Kuranaga, E, Takemoto, K, Hiratou, T, Okano, H & Miura, M 2005, 'Drosophila caspase transduces Shaggy/GSK-3β kinase activity in neural precursor development', EMBO Journal, vol. 24, no. 21, pp. 3793-3806. https://doi.org/10.1038/sj.emboj.7600822
Kanuka, Hirotaka ; Kuranaga, Erina ; Takemoto, Kiwamu ; Hiratou, Tetsuo ; Okano, Hideyuki ; Miura, Masayuki. / Drosophila caspase transduces Shaggy/GSK-3β kinase activity in neural precursor development. In: EMBO Journal. 2005 ; Vol. 24, No. 21. pp. 3793-3806.
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