Drosophila deltex mediates suppressor of hairless-independent and late-endosomal activation of notch signaling

Kazuya Hori, Maggy Fostier, Mikiko Ito, Takashi J. Fuwa, Masahiro J. Go, Hideyuki Okano, Martin Baron, Kenji Matsuno

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Notch (N) signaling is an evolutionarily conserved mechanism that regulates many cell-fate decisions. deltex (dx) encodes an E3-ubiquitin ligase that binds to the intracellular domain of N and positively regulates N signaling. However, the precise mechanism of Dx action is unknown. Here, we found that Dx was required and sufficient to activate the expression of gene targets of the canonical Su(H)-dependent N signaling pathway. Although Dx required N and a cis-acting element that overlaps with the Su(H)-binding site, Dx activated a target enhancer of N signaling, the dorsoventral compartment boundary enhancer of vestigial (vgBE), in a manner that was independent of the Delta (DI)/Serrate (Ser) ligands- or Su(H). Dx caused N to be moved from the apical cell surface into the late-endosome, where it accumulated stably and co-localized with Dx. Consistent with this, the dx gene was required for the presence of N in the endocytic vesicles. Finally, blocking the N transportation from the plasma membrane to the late-endosome by a dominant-negative form of Rab5 inhibited the Dx-mediated activation of N signaling, suggesting that the accumulation of N in the late-endosome was required for the Dx-mediated Su(H)-independent N signaling.

Original languageEnglish
Pages (from-to)5527-5537
Number of pages11
JournalDevelopment
Volume131
Issue number22
DOIs
Publication statusPublished - 2004 Nov

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Endosomes
Drosophila
Transport Vesicles
Ubiquitin-Protein Ligases
Binding Sites
Cell Membrane
Ligands
Gene Expression
Genes

Keywords

  • Deltex
  • Drosophila
  • Endocytic trafficking
  • Notch signaling
  • Suppressor of hairless

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Drosophila deltex mediates suppressor of hairless-independent and late-endosomal activation of notch signaling. / Hori, Kazuya; Fostier, Maggy; Ito, Mikiko; Fuwa, Takashi J.; Go, Masahiro J.; Okano, Hideyuki; Baron, Martin; Matsuno, Kenji.

In: Development, Vol. 131, No. 22, 11.2004, p. 5527-5537.

Research output: Contribution to journalArticle

Hori, K, Fostier, M, Ito, M, Fuwa, TJ, Go, MJ, Okano, H, Baron, M & Matsuno, K 2004, 'Drosophila deltex mediates suppressor of hairless-independent and late-endosomal activation of notch signaling', Development, vol. 131, no. 22, pp. 5527-5537. https://doi.org/10.1242/dev.01448
Hori, Kazuya ; Fostier, Maggy ; Ito, Mikiko ; Fuwa, Takashi J. ; Go, Masahiro J. ; Okano, Hideyuki ; Baron, Martin ; Matsuno, Kenji. / Drosophila deltex mediates suppressor of hairless-independent and late-endosomal activation of notch signaling. In: Development. 2004 ; Vol. 131, No. 22. pp. 5527-5537.
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AU - Baron, Martin

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