DrRad51 is required for chiasmata formation in meiosis in planarian Dugesia ryukyuensis

Ayako Chinone, Midori Matsumoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

SUMMARY: Rad51, a conserved eukaryotic protein, mediates the homologous-recombination repair of DNA double-strand breaks that occur during both mitosis and meiosis. During prophase I of meiosis, homologous recombination enhances the linkage between homologous chromosomes to increase the accuracy of segregation at anaphase I. In polyploidy situations, however, difficulties with homologous chromosome segregation often disrupt meiosis. Yet, triploid individuals of the planarian Dugesia ryukyuensis are able to produce functional gametes through a specialized form of meiosis. To shed light on the molecular mechanisms that promote successful meiosis in triploid D. ryukyuensis, we investigated rad51 gene function. We isolated three genes of the Rad51 family, the Rad51 homolog Dr-rad51 and the Rad51 paralogs Dr-rad51B and Dr-rad51C. Dr-rad51 was expressed in germ-line and presumably in somatic stem cells, but was not necessary for the regeneration of somatic tissue. RNA-interference (RNAi) depletion of Dr-rad51 during sexualization did not affect chromosome behavior in zygotene oocytes, but did result in the loss of chiasmata at the diplotene stage. Thus, homologous recombination does not appear to be necessary for synapsis, but is needed for crossover and proper segregation in D. ryukyuensis.

Original languageEnglish
Pages (from-to)409-421
Number of pages13
JournalMolecular Reproduction and Development
Volume81
Issue number5
DOIs
Publication statusPublished - 2014

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Planarians
Meiosis
Meiotic Prophase I
Triploidy
Homologous Recombination
Germ Cells
Chromosomes
Chromosome Pairing
Recombinational DNA Repair
Chromosome Segregation
Anaphase
Adult Stem Cells
Polyploidy
RNA Interference
Mitosis
Genes
Oocytes
Regeneration
Proteins

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology
  • Medicine(all)

Cite this

DrRad51 is required for chiasmata formation in meiosis in planarian Dugesia ryukyuensis. / Chinone, Ayako; Matsumoto, Midori.

In: Molecular Reproduction and Development, Vol. 81, No. 5, 2014, p. 409-421.

Research output: Contribution to journalArticle

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AB - SUMMARY: Rad51, a conserved eukaryotic protein, mediates the homologous-recombination repair of DNA double-strand breaks that occur during both mitosis and meiosis. During prophase I of meiosis, homologous recombination enhances the linkage between homologous chromosomes to increase the accuracy of segregation at anaphase I. In polyploidy situations, however, difficulties with homologous chromosome segregation often disrupt meiosis. Yet, triploid individuals of the planarian Dugesia ryukyuensis are able to produce functional gametes through a specialized form of meiosis. To shed light on the molecular mechanisms that promote successful meiosis in triploid D. ryukyuensis, we investigated rad51 gene function. We isolated three genes of the Rad51 family, the Rad51 homolog Dr-rad51 and the Rad51 paralogs Dr-rad51B and Dr-rad51C. Dr-rad51 was expressed in germ-line and presumably in somatic stem cells, but was not necessary for the regeneration of somatic tissue. RNA-interference (RNAi) depletion of Dr-rad51 during sexualization did not affect chromosome behavior in zygotene oocytes, but did result in the loss of chiasmata at the diplotene stage. Thus, homologous recombination does not appear to be necessary for synapsis, but is needed for crossover and proper segregation in D. ryukyuensis.

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