Drug contact time dominates a necessary time for myocardial cells necrosis by a photodynamic reaction

Emiyu Ogawa, Yuiko Kikuchi, Hiroshi Kumagai, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We studied the necessary time for cell necrosis on myocardial cells against a photodynamic reaction using talaporfin sodium with various drug contact time in vitro. We have developed a new methodology of cardiac catheter ablation for tachyarrhythmia using a photodynamic reaction. To realize an immediate electrical conduction block within a few minutes, we intended to occur the photodynamic reaction outside the cells by using a short drug contact time. In a clinical situation, the drug distribution around the cells changes continuously after the drug administration. To study the change of electrical conduction block performance with these drug distribution change, we measured the necessary time for myocardial cell necrosis with various drug contact time. The concentration of intracellular Ca 2+ ion was measured by Fluo-4 AM fluorescence dye during the photodynamic reaction with excitation light of 663 nm in wavelength under a confocal microscope. The time for myocardial cell necrosis was evaluated using a decrease of Ca 2+ ion after the cell membrane rupture. The drug contact time for myocardial cells was varied 1-60 min. We obtained that the necessary time for myocardial cell decreased until around 10 min of drug contact time, and it increased after around 20 min of drug contact time. We suggest that the drug contact time within 10-20 min would the most effective timing to minimize the necessary time for myocardial cell necrosis using photodynamic reaction since the oxidative stress during uptake process would be high.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXX
EditorsBennett L. Ibey, Hope Thomas Beier
PublisherSPIE
ISBN (Electronic)9781510623941
DOIs
Publication statusPublished - 2019 Jan 1
EventOptical Interactions with Tissue and Cells XXX 2019 - San Francisco, United States
Duration: 2019 Feb 22019 Feb 3

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10876
ISSN (Print)1605-7422

Conference

ConferenceOptical Interactions with Tissue and Cells XXX 2019
CountryUnited States
CitySan Francisco
Period19/2/219/2/3

Keywords

  • Cardiomyocyte
  • Catheter ablation
  • Cell death
  • Drug-light interval
  • Electrical conduction
  • Photosensitization reaction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Ogawa, E., Kikuchi, Y., Kumagai, H., & Arai, T. (2019). Drug contact time dominates a necessary time for myocardial cells necrosis by a photodynamic reaction. In B. L. Ibey, & H. T. Beier (Eds.), Optical Interactions with Tissue and Cells XXX [1087602] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10876). SPIE. https://doi.org/10.1117/12.2508112