Drying and extraction effects on three edible brown seaweeds for metabolomics

Shahlizah Sahul Hamid, Masataka Wakayama, Tomoyoshi Soga, Masaru Tomita

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Metabolomics is often used to comprehensively elucidate the metabolites in organisms like seaweed. Amino acids hydrolysed from proteins and certain targeted metabolites in seaweed have been investigated. However, water-soluble metabolites like free amino acids, organic acids, and sugars have seldom been comprehensively analysed. Metabolomics are valuable tools for these studies, but they require optimisation of pre-treatment methodology. Here, we evaluated various pre-treatment drying and extraction methods for brown seaweed metabolomics. Three edible brown seaweeds (Cladosiphon okamuranus [Mozuku], Saccharina japonica [Kombu], and Undaria pinnatifida [Wakame]) were used. Freeze-drying and oven-drying at both 40 and 80 °C were investigated. Methanol-water extracts with and without chloroform were compared. Metabolites were evaluated and quantified using liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry. The results showed that metabolite profiling was determined mainly by seaweed species identity rather than pre-treatment method. Freeze-drying yielded higher metabolite concentrations than oven-drying at either 40 or 80 °C. The effects of extraction with and without chloroform on metabolite concentration varied with seaweed species.

Original languageEnglish
JournalJournal of Applied Phycology
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Phaeophyceae
metabolomics
seaweed
metabolite
drying
metabolites
macroalgae
Undaria pinnatifida
freeze drying
pretreatment
chloroform
ovens
amino acid
mass spectrometry
Saccharina japonica
capillary electrophoresis
effect
extraction method
organic acid
free amino acids

Keywords

  • Brown seaweed
  • Drying methods
  • Extraction methods
  • Metabolomics
  • Phaeophyceae

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Drying and extraction effects on three edible brown seaweeds for metabolomics. / Hamid, Shahlizah Sahul; Wakayama, Masataka; Soga, Tomoyoshi; Tomita, Masaru.

In: Journal of Applied Phycology, 01.01.2018.

Research output: Contribution to journalArticle

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