Dual effects of n-alcohols on fluid secretion from guinea pig pancreatic ducts

Hiroyuki Hamada, Hiroshi Ishiguro, Akiko Yamamoto, Sachiko Shimano-Futakuchi, Shigeru Ko, Toshiyuki Yoshikawa, Hidemi Goto, Motoji Kitagawa, Tetsuo Hayakawa, Yoshiteru Seo, Satoru Naruse

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ethanol strongly augments secretin-stimulated, but not acetylcholine (ACh)-stimulated, fluid secretion from pancreatic duct cells. To understand its mechanism of action, we examined the effect of short-chain n-alcohols on fluid secretion and intracellular Ca2+ concentration ([Ca 2+]i) in guinea pig pancreatic ducts. Fluid secretion was measured by monitoring the luminal volume of isolated interlobular ducts. [Ca2+]i was estimated using fura-2 microfluorometry. Methanol and ethanol at 0.3-10 mM concentrations significantly augmented fluid secretion and induced a transient elevation of [Ca2+]i in secretin- or dibutyryl adenosine 3′,5′-cyclic monophosphate (DBcAMP)-stimulated ducts. However, they failed to affect fluid secretion and [Ca2+]i in unstimulated and ACh-stimulated ducts. In contrast, propanol and butanol at 0.3-10 mM concentrations significantly reduced fluid secretion and decreased [Ca2+]i in unstimulated ducts and in ducts stimulated with secretin, DBcAMP, or ACh. Both stimulatory and inhibitory effects of n-alcohols completely disappeared after their removal from the perfusate. Propanol and butanol inhibited the plateau phase, but not the initial peak, of [Ca2+]i response to ACh as well as the [Ca2+]i elevation induced by thapsigargin, suggesting that they inhibit Ca2+ influx. Removal of extracellular Ca 2+ reduced [Ca2+]i in duct cells and completely abolished secretin-stimulated fluid secretion. In conclusion, there is a distinct cutoff point between ethanol (C2) and propanol (C3) in their effects on fluid secretion and [Ca2+]i in duct cells. Short-chain n-alcohols appear to affect pancreatic ductal fluid secretion by activating or inhibiting the plasma membrane Ca2+ channel.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume288
Issue number6 57-6
DOIs
Publication statusPublished - 2005 Jun
Externally publishedYes

Fingerprint

Fluids and Secretions
Pancreatic Ducts
Ducts
Guinea Pigs
Alcohols
Fluids
Secretin
1-Propanol
Acetylcholine
Bucladesine
Butanols
Ethanol
Cytophotometry
Thapsigargin
Fura-2
Cell membranes
Adenosine
Methanol
Cell Membrane

Keywords

  • Acetylcholine
  • Intracellular calcium

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Hamada, H., Ishiguro, H., Yamamoto, A., Shimano-Futakuchi, S., Ko, S., Yoshikawa, T., ... Naruse, S. (2005). Dual effects of n-alcohols on fluid secretion from guinea pig pancreatic ducts. American Journal of Physiology - Cell Physiology, 288(6 57-6). https://doi.org/10.1152/ajpcell.00373.2004

Dual effects of n-alcohols on fluid secretion from guinea pig pancreatic ducts. / Hamada, Hiroyuki; Ishiguro, Hiroshi; Yamamoto, Akiko; Shimano-Futakuchi, Sachiko; Ko, Shigeru; Yoshikawa, Toshiyuki; Goto, Hidemi; Kitagawa, Motoji; Hayakawa, Tetsuo; Seo, Yoshiteru; Naruse, Satoru.

In: American Journal of Physiology - Cell Physiology, Vol. 288, No. 6 57-6, 06.2005.

Research output: Contribution to journalArticle

Hamada, H, Ishiguro, H, Yamamoto, A, Shimano-Futakuchi, S, Ko, S, Yoshikawa, T, Goto, H, Kitagawa, M, Hayakawa, T, Seo, Y & Naruse, S 2005, 'Dual effects of n-alcohols on fluid secretion from guinea pig pancreatic ducts', American Journal of Physiology - Cell Physiology, vol. 288, no. 6 57-6. https://doi.org/10.1152/ajpcell.00373.2004
Hamada, Hiroyuki ; Ishiguro, Hiroshi ; Yamamoto, Akiko ; Shimano-Futakuchi, Sachiko ; Ko, Shigeru ; Yoshikawa, Toshiyuki ; Goto, Hidemi ; Kitagawa, Motoji ; Hayakawa, Tetsuo ; Seo, Yoshiteru ; Naruse, Satoru. / Dual effects of n-alcohols on fluid secretion from guinea pig pancreatic ducts. In: American Journal of Physiology - Cell Physiology. 2005 ; Vol. 288, No. 6 57-6.
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abstract = "Ethanol strongly augments secretin-stimulated, but not acetylcholine (ACh)-stimulated, fluid secretion from pancreatic duct cells. To understand its mechanism of action, we examined the effect of short-chain n-alcohols on fluid secretion and intracellular Ca2+ concentration ([Ca 2+]i) in guinea pig pancreatic ducts. Fluid secretion was measured by monitoring the luminal volume of isolated interlobular ducts. [Ca2+]i was estimated using fura-2 microfluorometry. Methanol and ethanol at 0.3-10 mM concentrations significantly augmented fluid secretion and induced a transient elevation of [Ca2+]i in secretin- or dibutyryl adenosine 3′,5′-cyclic monophosphate (DBcAMP)-stimulated ducts. However, they failed to affect fluid secretion and [Ca2+]i in unstimulated and ACh-stimulated ducts. In contrast, propanol and butanol at 0.3-10 mM concentrations significantly reduced fluid secretion and decreased [Ca2+]i in unstimulated ducts and in ducts stimulated with secretin, DBcAMP, or ACh. Both stimulatory and inhibitory effects of n-alcohols completely disappeared after their removal from the perfusate. Propanol and butanol inhibited the plateau phase, but not the initial peak, of [Ca2+]i response to ACh as well as the [Ca2+]i elevation induced by thapsigargin, suggesting that they inhibit Ca2+ influx. Removal of extracellular Ca 2+ reduced [Ca2+]i in duct cells and completely abolished secretin-stimulated fluid secretion. In conclusion, there is a distinct cutoff point between ethanol (C2) and propanol (C3) in their effects on fluid secretion and [Ca2+]i in duct cells. Short-chain n-alcohols appear to affect pancreatic ductal fluid secretion by activating or inhibiting the plasma membrane Ca2+ channel.",
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AU - Ko, Shigeru

AU - Yoshikawa, Toshiyuki

AU - Goto, Hidemi

AU - Kitagawa, Motoji

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AU - Naruse, Satoru

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