Dual responses of tissue partial pressure of oxygen after functional stimulation in rat somatosensory cortex

Kazuto Masamoto, Naosada Takizawa, Hirosuke Kobayashi, Kotaro Oka, Kazuo Tanishita

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

To compare the spatial heterogeneity of brain tissue partial pressure of oxygen (pO2) among local brain regions, we focused on functional and anatomical variations in rat somatosensory cortex. Tissue pO2 was measured by using an oxygen microelectrode with high spatio-temporal resolution, and investigated in three somatosensory areas including hindlimb (HL), forelimb (FL), and trunk region (Tr). Their anatomical structures were determined with histological techniques (Nissl stain). In addition to the measurement of baseline tissue pO2, we examined temporal shifts in tissue pO2 distribution elicited by functional stimulation using the brushing stimulation to the hindlimb, forelimb, and trunk regions of the body. We observed that average tissue pO2 in the Tr (14±10 Torr) was significantly lower than those in the HL (25±13 Torr) and FL (24±13 Torr). Such regional differences in tissue pO2 were closely related to the cytoarchitectonic variations among these three areas. In addition, the functional stimulation enlarged the regional differences in the pO2 depending on each somatosensory area; the pO2 in the HL increased by 3.6±2.9% after the stimulation to hindlimb, whereas that in the Tr decreased by -2.9±2.5% after the stimulation to trunk region. Such dual responses of tissue pO2 (i.e. increase or decrease) after the functional stimulation to the corresponding body regions may provide a criterion to clinically predict regions susceptible to tissue hypoxia, because considerable decrease in tissue pO2 occurred in the Tr showing the lowest baseline pO2.

Original languageEnglish
Pages (from-to)104-113
Number of pages10
JournalBrain Research
Volume979
Issue number1-2
DOIs
Publication statusPublished - 2003 Jul 25

Fingerprint

Somatosensory Cortex
Partial Pressure
Oxygen
Hindlimb
Forelimb
Body Regions
Histological Techniques
Brain
Microelectrodes
Tissue Distribution
Coloring Agents

Keywords

  • Functional stimulation
  • Oxygen microelectrode
  • Rat somatosensory cortex
  • Tissue pO

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dual responses of tissue partial pressure of oxygen after functional stimulation in rat somatosensory cortex. / Masamoto, Kazuto; Takizawa, Naosada; Kobayashi, Hirosuke; Oka, Kotaro; Tanishita, Kazuo.

In: Brain Research, Vol. 979, No. 1-2, 25.07.2003, p. 104-113.

Research output: Contribution to journalArticle

Masamoto, Kazuto ; Takizawa, Naosada ; Kobayashi, Hirosuke ; Oka, Kotaro ; Tanishita, Kazuo. / Dual responses of tissue partial pressure of oxygen after functional stimulation in rat somatosensory cortex. In: Brain Research. 2003 ; Vol. 979, No. 1-2. pp. 104-113.
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