Dual temperature-and pH-responsive fluorescence molecular probe for cellular imaging utilizing a PNIPAAm-fluorescein copolymer

Hiroki Kobayashi, Mayumi Nishikawa, Chikako Sakamoto, Tadashi Nishio, Hideko Kanazawa, Teruo Okano

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The thermally controlling fluorescence intensity of a molecular probe for cellular imaging has been investigated. A reversible temperature-induced phase transition of N-isopropylacrylamide/fluorescein copolymers [poly(NIPAAm-co-FL)] was used as a molecular switch to control the fluorescence intensity of the imaging probe. The copolymer displayed environmentally sensitive fluorescence properties, in which the fluorescence intensity changed with the response to both the temperature and the pH. Utilizing these features, we monitored the thermal-aggregation process of BSA by the fluorescence resonance energy transfer (FRET) method. Additionally, the cellular uptake in RAW264.7 cells of poly(NIPAAm-co-FL) conjugated with lipid was studied using a confocal laser scanning microscope. 2009

Original languageEnglish
Pages (from-to)1043-1047
Number of pages5
JournalAnalytical Sciences
Volume25
Issue number8
DOIs
Publication statusPublished - 2009 Aug

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Molecular Probes
Fluorescein
Copolymers
Fluorescence
Imaging techniques
Temperature
Microscopes
Agglomeration
Phase transitions
Switches
Scanning
Lipids
poly-N-isopropylacrylamide
Lasers
poly(NIPAAm-co-FL)

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Dual temperature-and pH-responsive fluorescence molecular probe for cellular imaging utilizing a PNIPAAm-fluorescein copolymer. / Kobayashi, Hiroki; Nishikawa, Mayumi; Sakamoto, Chikako; Nishio, Tadashi; Kanazawa, Hideko; Okano, Teruo.

In: Analytical Sciences, Vol. 25, No. 8, 08.2009, p. 1043-1047.

Research output: Contribution to journalArticle

Kobayashi, Hiroki ; Nishikawa, Mayumi ; Sakamoto, Chikako ; Nishio, Tadashi ; Kanazawa, Hideko ; Okano, Teruo. / Dual temperature-and pH-responsive fluorescence molecular probe for cellular imaging utilizing a PNIPAAm-fluorescein copolymer. In: Analytical Sciences. 2009 ; Vol. 25, No. 8. pp. 1043-1047.
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