Duck-billed platypus venom peptides induce Ca2+ influx in neuroblastoma cells

Masaki Kita, David St C. Black, Osamu Ohno, Kaoru Yamada, Hideo Kigoshi, Daisuke Uemura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

(Chemical Equation Presented) The duck-billed platypus (Ornithorhynchus anatinus) is one of the few venomous Australian mammals. We previously found that its crude venom potently induces Ca2+ influx in human neuroblastoma IMR-32 cells. Guided by this bioassay, we identified 11 novel peptides, including the heptapeptide H-His-Asp-His-Pro-Asn-Pro-Arg-OH (1). Compounds 1-4 and 5-11 coincided with the 6-9 N-terminal residues of Ornithorhynchus venom C-type natriuretic peptide (OvCNP) and the 132-150 part of OvCNP precursor peptide, respectively. Heptapeptide 1, which is one of the primary components of the venom fluid ( 200 ng/μL), induced a significant increase in [Ca2+]i in IMR-32 cells at 75 μM. To the best of our knowledge, this is the first example of the isolation of the N-terminal linear fragments of CNPs in any mammal.

Original languageEnglish
Pages (from-to)18038-18039
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number50
DOIs
Publication statusPublished - 2009 Dec 23

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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    Kita, M., Black, D. S. C., Ohno, O., Yamada, K., Kigoshi, H., & Uemura, D. (2009). Duck-billed platypus venom peptides induce Ca2+ influx in neuroblastoma cells. Journal of the American Chemical Society, 131(50), 18038-18039. https://doi.org/10.1021/ja908148z