Duck-billed platypus venom peptides induce Ca2+ influx in neuroblastoma cells

Masaki Kita, David StC Black, Osamu Ohno, Kaoru Yamada, Hideo Kigoshi, Daisuke Uemura

Research output: Contribution to journalArticle

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Abstract

(Chemical Equation Presented) The duck-billed platypus (Ornithorhynchus anatinus) is one of the few venomous Australian mammals. We previously found that its crude venom potently induces Ca2+ influx in human neuroblastoma IMR-32 cells. Guided by this bioassay, we identified 11 novel peptides, including the heptapeptide H-His-Asp-His-Pro-Asn-Pro-Arg-OH (1). Compounds 1-4 and 5-11 coincided with the 6-9 N-terminal residues of Ornithorhynchus venom C-type natriuretic peptide (OvCNP) and the 132-150 part of OvCNP precursor peptide, respectively. Heptapeptide 1, which is one of the primary components of the venom fluid ( 200 ng/μL), induced a significant increase in [Ca2+]i in IMR-32 cells at 75 μM. To the best of our knowledge, this is the first example of the isolation of the N-terminal linear fragments of CNPs in any mammal.

Original languageEnglish
Pages (from-to)18038-18039
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number50
DOIs
Publication statusPublished - 2009 Dec 23

Fingerprint

Platypus
Mammals
Ducks
Venoms
Neuroblastoma
Peptides
C-Type Natriuretic Peptide
Bioassay
Fluids
Biological Assay
Natriuretic Peptides

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Kita, M., Black, D. S., Ohno, O., Yamada, K., Kigoshi, H., & Uemura, D. (2009). Duck-billed platypus venom peptides induce Ca2+ influx in neuroblastoma cells. Journal of the American Chemical Society, 131(50), 18038-18039. https://doi.org/10.1021/ja908148z

Duck-billed platypus venom peptides induce Ca2+ influx in neuroblastoma cells. / Kita, Masaki; Black, David StC; Ohno, Osamu; Yamada, Kaoru; Kigoshi, Hideo; Uemura, Daisuke.

In: Journal of the American Chemical Society, Vol. 131, No. 50, 23.12.2009, p. 18038-18039.

Research output: Contribution to journalArticle

Kita, M, Black, DS, Ohno, O, Yamada, K, Kigoshi, H & Uemura, D 2009, 'Duck-billed platypus venom peptides induce Ca2+ influx in neuroblastoma cells', Journal of the American Chemical Society, vol. 131, no. 50, pp. 18038-18039. https://doi.org/10.1021/ja908148z
Kita, Masaki ; Black, David StC ; Ohno, Osamu ; Yamada, Kaoru ; Kigoshi, Hideo ; Uemura, Daisuke. / Duck-billed platypus venom peptides induce Ca2+ influx in neuroblastoma cells. In: Journal of the American Chemical Society. 2009 ; Vol. 131, No. 50. pp. 18038-18039.
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