Dynactin is essential for growth cone advance

Takako K. Abe; Takao Honda; Kohtaro Takei; Katsuhiko Mikoshiba; Diane Hoffman-Kim; Daniel G. Jay; Ryozo Kuwano

doi:10.1016/j.bbrc.2008.05.008

Dynactin is essential for growth cone advance

Takako K. Abe, Takao Honda, Kohtaro Takei, Katsuhiko Mikoshiba, Diane Hoffman-Kim, Daniel G. Jay, Ryozo Kuwano

Department of Anatomy

Research output: Contribution to journal › Article

12 Citations (Scopus)

Abstract

Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150^Glued, from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150^Glued, plays an essential role in growth cone advance.

Original language	English
Pages (from-to)	418-422
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	372
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2008.05.008
Publication status	Published - 2008 Aug 1

Keywords

Axon guidance
CALI (chromophore-assisted laser inactivation)
Dynactin
Growth cone motility
Neurite extension

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Abe, T. K., Honda, T., Takei, K., Mikoshiba, K., Hoffman-Kim, D., Jay, D. G., & Kuwano, R. (2008). Dynactin is essential for growth cone advance. Biochemical and Biophysical Research Communications, 372(3), 418-422. https://doi.org/10.1016/j.bbrc.2008.05.008

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abstract = "Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150Glued, from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150Glued, plays an essential role in growth cone advance.",

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AU - Abe, Takako K.

AU - Honda, Takao

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AU - Mikoshiba, Katsuhiko

AU - Hoffman-Kim, Diane

AU - Jay, Daniel G.

AU - Kuwano, Ryozo

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