TY - JOUR
T1 - Dynactin is essential for growth cone advance
AU - Abe, Takako K.
AU - Honda, Takao
AU - Takei, Kohtaro
AU - Mikoshiba, Katsuhiko
AU - Hoffman-Kim, Diane
AU - Jay, Daniel G.
AU - Kuwano, Ryozo
N1 - Funding Information:
This work was supported by Grants (to R.K.) from the Ministry of Education, Culture Science, Sports, and Technology, Japan. We thank Drs. Alian Xu and Feng-Song Wang for helpful discussions, and Dr. Teruo Abe for critical comment for the manuscript.
PY - 2008/8/1
Y1 - 2008/8/1
N2 - Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150Glued, from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150Glued, plays an essential role in growth cone advance.
AB - Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150Glued, from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150Glued, plays an essential role in growth cone advance.
KW - Axon guidance
KW - CALI (chromophore-assisted laser inactivation)
KW - Dynactin
KW - Growth cone motility
KW - Neurite extension
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U2 - 10.1016/j.bbrc.2008.05.008
DO - 10.1016/j.bbrc.2008.05.008
M3 - Article
C2 - 18477476
AN - SCOPUS:44749088806
VL - 372
SP - 418
EP - 422
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -