Abstract
Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150Glued, from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150Glued, plays an essential role in growth cone advance.
| Original language | English |
|---|---|
| Pages (from-to) | 418-422 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 372 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2008 Aug 1 |
| Externally published | Yes |
Fingerprint
Keywords
- Axon guidance
- CALI (chromophore-assisted laser inactivation)
- Dynactin
- Growth cone motility
- Neurite extension
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Dynactin is essential for growth cone advance. / Abe, Takako K.; Honda, Takao; Takei, Kohtaro; Mikoshiba, Katsuhiko; Hoffman-Kim, Diane; Jay, Daniel G.; Kuwano, Ryozo.
In: Biochemical and Biophysical Research Communications, Vol. 372, No. 3, 01.08.2008, p. 418-422.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynactin is essential for growth cone advance
AU - Abe, Takako K.
AU - Honda, Takao
AU - Takei, Kohtaro
AU - Mikoshiba, Katsuhiko
AU - Hoffman-Kim, Diane
AU - Jay, Daniel G.
AU - Kuwano, Ryozo
PY - 2008/8/1
Y1 - 2008/8/1
N2 - Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150Glued, from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150Glued, plays an essential role in growth cone advance.
AB - Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150Glued, from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150Glued, plays an essential role in growth cone advance.
KW - Axon guidance
KW - CALI (chromophore-assisted laser inactivation)
KW - Dynactin
KW - Growth cone motility
KW - Neurite extension
UR - http://www.scopus.com/inward/record.url?scp=44749088806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44749088806&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.05.008
DO - 10.1016/j.bbrc.2008.05.008
M3 - Article
C2 - 18477476
AN - SCOPUS:44749088806
VL - 372
SP - 418
EP - 422
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -