Abstract
Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150Glued, from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150Glued, plays an essential role in growth cone advance.
Original language | English |
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Pages (from-to) | 418-422 |
Number of pages | 5 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 372 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2008 Aug 1 |
Externally published | Yes |
Keywords
- Axon guidance
- CALI (chromophore-assisted laser inactivation)
- Dynactin
- Growth cone motility
- Neurite extension
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology