Dynamic diameter response of intraparenchymal penetrating arteries during cortical spreading depression and elimination of vasoreactivity to hypercapnia in anesthetized mice

Miyuki Unekawa, Yutaka Tomita, Kazuto Masamoto, Haruki Toriumi, Takashi Osada, Iwao Kanno, Norihiro Suzuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cortical spreading depression (CSD) induces marked hyperemia with a transient decrease of regional cerebral blood flow (rCBF), followed by sustained oligemia. To further understand the microcirculatory mechanisms associated with CSD, we examined the temporal changes of diameter of intraparenchymal penetrating arteries during CSD. In urethane-anesthetized mice, the diameter of single penetrating arteries at three depths was measured using two-photon microscopy during passage of repeated CSD, with continuous recordings of direct current potential and rCBF. The first CSD elicited marked constriction superimposed on the upstrokes of profound dilation throughout each depth of the penetrating artery, and the vasoreaction temporally corresponded to the change of rCBF. Second or later CSD elicited marked dilation with little or no constriction phase throughout each depth, and the vasodilation also temporally corresponded to the increase of rCBF. Furthermore, the peak dilation showed good negative correlations with basal diameter and increase of rCBF. Vasodilation induced by 5% CO 2 inhalation was significantly suppressed after CSD passage at any depth as well as hyperperfusion. These results may indicate that CSD-induced rCBF changes mainly reflect the diametric changes of the intraparenchymal arteries, despite the elimination of responsiveness to hypercapnia.

Original languageEnglish
Pages (from-to)657-670
Number of pages14
JournalJournal of Cerebral Blood Flow and Metabolism
Volume37
Issue number2
DOIs
Publication statusPublished - 2016

Fingerprint

Cortical Spreading Depression
Cerebrovascular Circulation
Hypercapnia
Regional Blood Flow
Arteries
Dilatation
Constriction
Vasodilation
Urethane
Hyperemia
Carbon Monoxide
Photons
Inhalation
Microscopy

Keywords

  • Cortical spreading depression
  • hypercapnia
  • microcirculation
  • penetrating artery
  • two-photon microscopy

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Dynamic diameter response of intraparenchymal penetrating arteries during cortical spreading depression and elimination of vasoreactivity to hypercapnia in anesthetized mice. / Unekawa, Miyuki; Tomita, Yutaka; Masamoto, Kazuto; Toriumi, Haruki; Osada, Takashi; Kanno, Iwao; Suzuki, Norihiro.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 37, No. 2, 2016, p. 657-670.

Research output: Contribution to journalArticle

Unekawa, Miyuki ; Tomita, Yutaka ; Masamoto, Kazuto ; Toriumi, Haruki ; Osada, Takashi ; Kanno, Iwao ; Suzuki, Norihiro. / Dynamic diameter response of intraparenchymal penetrating arteries during cortical spreading depression and elimination of vasoreactivity to hypercapnia in anesthetized mice. In: Journal of Cerebral Blood Flow and Metabolism. 2016 ; Vol. 37, No. 2. pp. 657-670.
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