Dynamic Flow Velocity Mapping from Fluorescent Dye Transit Times in the Brain Surface Microcirculation of Anesthetized Rats and Mice

Ryo Hoshikawa, Hiroshi Kawaguchi, Hiroyuki Takuwa, Yoko Ikoma, Yutaka Tomita, Miyuki Unekawa, Norihiro Suzuki, Iwao Kanno, Kazuto Masamoto

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Objective: This study aimed to develop a new method for mapping blood flow velocity based on the spatial evolution of fluorescent dye transit times captured with CLSFM in the cerebral microcirculation of anesthetized rodents. Methods: The animals were anesthetized with isoflurane, and a small amount of fluorescent dye was intravenously injected to label blood plasma. The CLSFM was conducted through a closed cranial window to capture propagation of the dye in the cortical vessels. The transit time of the dye over a certain distance in a single vessel was determined with automated image analyses, and average flow velocity was mapped in each vessel. Results: The average flow velocity measured in the rat pial artery and vein was 4.4 ± 1.2 and 2.4 ± 0.5 mm/sec, respectively. A similar range of flow velocity to those of the rats was observed in the mice; 4.9 ± 1.4 and 2.0 ± 0.9 mm/sec, respectively, although the vessel diameter in the mice was about half of that in the rats. Conclusions: Flow velocity in the cerebral microcirculation can be mapped based on fluorescent dye transit time measurements with conventional CLSFM in experimental animals.

Original languageEnglish
Pages (from-to)416-425
Number of pages10
JournalMicrocirculation
Volume23
Issue number6
DOIs
Publication statusPublished - 2016 Aug 1

Keywords

  • capillary heterogeneity
  • cerebral blood flow
  • dilution technique
  • fluorescent imaging
  • neurovascular unit

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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