Dynamic interactions between a membrane binding protein and lipids induce fluctuating diffusivity

Eiji Yamamoto, Takuma Akimoto, Antreas C. Kalli, Kenji Yasuoka, Mark S.P. Sansom

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Pleckstrin homology (PH) domains are membrane-binding lipid recognition proteins that interact with phosphatidylinositol phosphate (PIP) molecules in eukaryotic cell membranes. Diffusion of PH domains plays a critical role in biological reactions on membrane surfaces. Although diffusivity can be estimated by long-time measurements, it lacks information on the short-time diffusive nature. We reveal two diffusive properties of a PH domain bound to the surface of a PIP-containing membrane using molecular dynamics simulations. One is fractional Brownian motion, attributed to the motion of the lipids with which the PH domain interacts. The other is temporally fluctuating diffusivity; that is, the short-time diffusivity of the bound protein changes substantially with time. Moreover, the diffusivity for short-time measurements is intrinsically different from that for long-time measurements. This fluctuating diffusivity results from dynamic changes in interactions between the PH domain and PIP molecules. Our results provide evidence that the complexity of protein-lipid interactions plays a crucial role in the diffusion of proteins on biological membrane surfaces. Changes in the diffusivity of PH domains and related membrane-bound proteins may in turn contribute to the formation/dissolution of protein complexes in membranes.

Original languageEnglish
Article numbere1601871
JournalScience Advances
Volume3
Issue number1
DOIs
Publication statusPublished - 2017 Jan

ASJC Scopus subject areas

  • General

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