Dynamic simulation of an in vitro multi-enzyme system

Nobuyoshi Ishii, Yoshihiro Suga, Akiko Hagiya, Hisami Watanabe, Hirotada Mori, Masataka Yoshino, Masaru Tomita

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Parameters often are tuned with metabolite concentration time series data to build a dynamic model of metabolism. However, such tuning may reduce the extrapolation ability (generalization capability) of the model. In this study, we determined detailed kinetic parameters of three purified Escherichia coli glycolytic enzymes using the initial velocity method for individual enzymes; i.e., the parameters were determined independently from metabolite concentration time series data. The metabolite concentration time series calculated by the model using the parameters matched the experimental data obtained in an actual multi-enzyme system consisting of the three purified E. coli glycolytic enzymes. Thus, the results indicate that kinetic parameters can be determined without using an undesirable tuning process.

Original languageEnglish
Pages (from-to)413-420
Number of pages8
JournalFEBS Letters
Volume581
Issue number3
DOIs
Publication statusPublished - 2007 Feb 6

Keywords

  • Dynamic simulation
  • Enzyme kinetics
  • Glycolysis
  • Mathematical model
  • Metabolism

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Ishii, N., Suga, Y., Hagiya, A., Watanabe, H., Mori, H., Yoshino, M., & Tomita, M. (2007). Dynamic simulation of an in vitro multi-enzyme system. FEBS Letters, 581(3), 413-420. https://doi.org/10.1016/j.febslet.2006.12.049