Dynamic subcellular localization of aquaporin-7 in white adipocytes

Takayuki Miyauchi, Hiroyuki Yamamoto, Yoichiro Abe, Go J. Yoshida, Aleksandra Rojek, Eisei Sohara, Shinichi Uchida, Søren Nielsen, Masato Yasui

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aquaporin-7 (AQP7) is expressed in adipose tissue, permeated by water and glycerol, and is involved in lipid metabolism. AQP7-null mice develop obesity, insulin resistance, and adipocyte hypertrophy. Here, we show that AQP7 is expressed in adipocyte plasma membranes, and is re-localized to intracellular membranes in response to catecholamine in mouse white adipose tissue. We found that internalization of AQP7 was induced by PKA activation and comparative gene identification 58 (CGI-58). This relocation was confirmed by functional studies in 3T3-L1 adipocytes. Collectively, these results suggest that AQP7 makes several contributions to adipocyte metabolism, in both cortical and intracellular membranes.

Original languageEnglish
Pages (from-to)608-614
Number of pages7
JournalFEBS Letters
Volume589
Issue number5
DOIs
Publication statusPublished - 2015 Feb 27

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Keywords

  • Adipocyte
  • Aquaporin-7
  • Comparative gene identification 58
  • Lipolysis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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