Dynamic subcellular localization of aquaporin-7 in white adipocytes

Takayuki Miyauchi, Hiroyuki Yamamoto, Yoichiro Abe, Go J. Yoshida, Aleksandra Rojek, Eisei Sohara, Shinichi Uchida, Søren Nielsen, Masato Yasui

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aquaporin-7 (AQP7) is expressed in adipose tissue, permeated by water and glycerol, and is involved in lipid metabolism. AQP7-null mice develop obesity, insulin resistance, and adipocyte hypertrophy. Here, we show that AQP7 is expressed in adipocyte plasma membranes, and is re-localized to intracellular membranes in response to catecholamine in mouse white adipose tissue. We found that internalization of AQP7 was induced by PKA activation and comparative gene identification 58 (CGI-58). This relocation was confirmed by functional studies in 3T3-L1 adipocytes. Collectively, these results suggest that AQP7 makes several contributions to adipocyte metabolism, in both cortical and intracellular membranes.

Original languageEnglish
Pages (from-to)608-614
Number of pages7
JournalFEBS Letters
Volume589
Issue number5
DOIs
Publication statusPublished - 2015 Feb 27

Fingerprint

White Adipocytes
Aquaporins
Adipocytes
Intracellular Membranes
Tissue
Membranes
White Adipose Tissue
Relocation
Cell membranes
Lipid Metabolism
Metabolism
Glycerol
Hypertrophy
Transcriptional Activation
Catecholamines
Insulin Resistance
Adipose Tissue
Obesity
Genes
Chemical activation

Keywords

  • Adipocyte
  • Aquaporin-7
  • Comparative gene identification 58
  • Lipolysis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology
  • Medicine(all)

Cite this

Dynamic subcellular localization of aquaporin-7 in white adipocytes. / Miyauchi, Takayuki; Yamamoto, Hiroyuki; Abe, Yoichiro; Yoshida, Go J.; Rojek, Aleksandra; Sohara, Eisei; Uchida, Shinichi; Nielsen, Søren; Yasui, Masato.

In: FEBS Letters, Vol. 589, No. 5, 27.02.2015, p. 608-614.

Research output: Contribution to journalArticle

Miyauchi, T, Yamamoto, H, Abe, Y, Yoshida, GJ, Rojek, A, Sohara, E, Uchida, S, Nielsen, S & Yasui, M 2015, 'Dynamic subcellular localization of aquaporin-7 in white adipocytes', FEBS Letters, vol. 589, no. 5, pp. 608-614. https://doi.org/10.1016/j.febslet.2015.01.025
Miyauchi, Takayuki ; Yamamoto, Hiroyuki ; Abe, Yoichiro ; Yoshida, Go J. ; Rojek, Aleksandra ; Sohara, Eisei ; Uchida, Shinichi ; Nielsen, Søren ; Yasui, Masato. / Dynamic subcellular localization of aquaporin-7 in white adipocytes. In: FEBS Letters. 2015 ; Vol. 589, No. 5. pp. 608-614.
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