Dynamics of global gene expression changes during mouse preimplantation development

Toshio Hamatani, Mark G. Carter, Alexei A. Sharov, Minoru S.H. Ko

Research output: Contribution to journalArticle

639 Citations (Scopus)

Abstract

Understanding preimplantation development is important both for basic reproductive biology and for practical applications including regenerative medicine and livestock breeding. Global expression profiles revealed and characterized the distinctive patterns of maternal RNA degradation and zygotic gene activation, including two major transient waves of de novo transcription. The first wave corresponds to zygotic genome activation (ZGA); the second wave, named mid-preimplantation gene activation (MGA), precedes the dynamic morphological and functional changes from the morula to blastocyst stage. Further expression profiling of embryos treated with inhibitors of transcription, translation, and DNA replication revealed that the translation of maternal RNAs is required for the initiation of ZGA. We propose a cascade of gene activation from maternal RNA/protein sets to ZGA gene sets and thence to MGA gene sets. The large number of genes identified as involved in each phase is a first step toward analysis of the complex gene regulatory networks.

Original languageEnglish
Pages (from-to)117-131
Number of pages15
JournalDevelopmental Cell
Volume6
Issue number1
DOIs
Publication statusPublished - 2004 Jan 1
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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