Dynein-dependent movement of autophagosomes mediates efficient encounters with lysosomes.

Shunsuke Kimura, Takeshi Noda, Tamotsu Yoshimori

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Autophagy is a membrane trafficking pathway that carries cytosolic components to the lysosome for degradation. During this process, the autophagosome, a double-membraned organelle, is generated de novo, sequesters cytoplasmic proteins and organelles, and delivers them to lysosomes. However, the mechanism by which autophagosomes are targeted to lysosomes has not been determined. Here, we observed the real-time behavior of microtubule-associated protein light chain 3 (LC3), which localizes to autophagosomes, and showed that autophagosomes move in a microtubule- and dynein-dynactin motor complex-dependent manner. After formation, autophagosomes show a rapid vectorial movement in the direction of the centrosome, where lysosomes are usually concentrated. Microinjection of antibodies against LC3 inhibited this movement; furthermore, using FRAP, we showed that anti-LC3 antibody injection caused a defect in targeting of autophagosomes to lysosomes. Collectively, our data demonstrate the functional significance of autophagosome movement that enables effective delivery from the cytosol to lysosomes.

Original languageEnglish
Pages (from-to)109-122
Number of pages14
JournalCell structure and function
Volume33
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1
Externally publishedYes

Fingerprint

Dyneins
Lysosomes
Light
Organelles
Centrosome
Microtubule-Associated Proteins
Antibodies
Autophagy
Microinjections
Autophagosomes
Microtubules
Cytosol
Injections
Membranes

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Dynein-dependent movement of autophagosomes mediates efficient encounters with lysosomes. / Kimura, Shunsuke; Noda, Takeshi; Yoshimori, Tamotsu.

In: Cell structure and function, Vol. 33, No. 1, 01.01.2008, p. 109-122.

Research output: Contribution to journalArticle

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