Dysfunctional gastric emptying with down-regulation of muscle-specific MicroRNAs in helicobacter pylori-infected mice

Yoshimasa Saito, Hidekazu Suzuki, Hitoshi Tsugawa, Sachiko Suzuki, Juntaro Matsuzaki, Kenro Hirata, Toshifumi Hibi

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background & Aims: Little is known about the pathogenic mechanisms of functional dyspepsia. We investigated the role of microRNAs (miRNAs) in gastric motility disorders associated with Helicobacter pylori infection. Methods: Male C57BL/6 mice were infected with H pylori. After long-term infection, gastric emptying was examined and compared with that of uninfected mice (controls). The miRNA expression profile was analyzed by miRNA microarray and quantitative reverse-transcriptase polymerase chain reaction. The results obtained from the animal study were confirmed by in vitro experiments. Results: Gastric emptying was significantly accelerated in mice after chronic infection with H pylori. Histologic examination showed that the muscular layers of the stomachs of H pylori-infected mice were significantly thickened. The miRNA expression profile revealed that the muscle-specific miRNAs miR-1 and miR-133 were significantly down-regulated in the stomachs after long-term infection with H pylori. However, expression of histone deacetylase 4 and serum response factor, which are reported target genes of miR-1 and miR-133, increased. Down-regulation of miR-1 and miR-133 and increased cell proliferation were observed in C2C12 mouse myoblast cells after coculture with H pylori. Conclusions:: Chronic infection with H pylori down-regulates expression of muscle-specific miRNAs and up-regulates expression of histone deacetylase 4 and serum response factor. These might cause hyperplasia in the muscular layer of the stomach and dysfunction in gastric emptying. These findings provide insight into the molecular pathogenesis of gastric motility disorders, including functional dyspepsia.

Original languageEnglish
Pages (from-to)189-198
Number of pages10
JournalGastroenterology
Volume140
Issue number1
DOIs
Publication statusPublished - 2011 Jan

Keywords

  • Functional Gastrointestinal Disorder
  • Liquid Gastric Emptying
  • Muscle Cell
  • Noncoding RNA

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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