Abstract
Embryonic stem (ES) cells cultured on gelatin-coated plates or feeder layers form tight aggregated colonies by the E-cadherin-mediated cell-cell adhesions. Here we show that murine ES cells do not make cell-cell contacts or form colonies when cultured on the plate coated with a fusion protein of E-cadherin and IgG Fc domain. The cells in culture retain all ES cell features including pluripotency to differentiate into cells of all three germ layers and germ-line transmission after extended culture. Furthermore, they show a higher proliferative ability, lower dependency on LIF, and higher transfection efficiency than colony-forming conditions. Our results suggest that aggregated colony formation might inhibit diffusion of soluble factors and increase cell-cell communication, which may result in a heterogeneous environment within and between surrounding cells of the colony. This method should enable more efficient and scalable culture of ES cells, an important step towards the clinical application of these cells.
| Original language | English |
|---|---|
| Article number | e15 |
| Journal | PLoS One |
| Volume | 1 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2006 Dec 20 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
E-cadherin-coated plates maintain pluripotent ES cells without colony formation. / Nagaoka, Masato; Koshimizu, Uichi; Yuasa, Shinsuke; Hattori, Fumiyuki; Chen, Hao; Tanaka, Tomofumi; Okabe, Masaru; Fukuda, Keiichi; Akaike, Toshihiro.
In: PLoS One, Vol. 1, No. 1, e15, 20.12.2006.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - E-cadherin-coated plates maintain pluripotent ES cells without colony formation
AU - Nagaoka, Masato
AU - Koshimizu, Uichi
AU - Yuasa, Shinsuke
AU - Hattori, Fumiyuki
AU - Chen, Hao
AU - Tanaka, Tomofumi
AU - Okabe, Masaru
AU - Fukuda, Keiichi
AU - Akaike, Toshihiro
PY - 2006/12/20
Y1 - 2006/12/20
N2 - Embryonic stem (ES) cells cultured on gelatin-coated plates or feeder layers form tight aggregated colonies by the E-cadherin-mediated cell-cell adhesions. Here we show that murine ES cells do not make cell-cell contacts or form colonies when cultured on the plate coated with a fusion protein of E-cadherin and IgG Fc domain. The cells in culture retain all ES cell features including pluripotency to differentiate into cells of all three germ layers and germ-line transmission after extended culture. Furthermore, they show a higher proliferative ability, lower dependency on LIF, and higher transfection efficiency than colony-forming conditions. Our results suggest that aggregated colony formation might inhibit diffusion of soluble factors and increase cell-cell communication, which may result in a heterogeneous environment within and between surrounding cells of the colony. This method should enable more efficient and scalable culture of ES cells, an important step towards the clinical application of these cells.
AB - Embryonic stem (ES) cells cultured on gelatin-coated plates or feeder layers form tight aggregated colonies by the E-cadherin-mediated cell-cell adhesions. Here we show that murine ES cells do not make cell-cell contacts or form colonies when cultured on the plate coated with a fusion protein of E-cadherin and IgG Fc domain. The cells in culture retain all ES cell features including pluripotency to differentiate into cells of all three germ layers and germ-line transmission after extended culture. Furthermore, they show a higher proliferative ability, lower dependency on LIF, and higher transfection efficiency than colony-forming conditions. Our results suggest that aggregated colony formation might inhibit diffusion of soluble factors and increase cell-cell communication, which may result in a heterogeneous environment within and between surrounding cells of the colony. This method should enable more efficient and scalable culture of ES cells, an important step towards the clinical application of these cells.
UR - http://www.scopus.com/inward/record.url?scp=55149084404&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55149084404&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0000015
DO - 10.1371/journal.pone.0000015
M3 - Article
C2 - 17183641
AN - SCOPUS:55149084404
VL - 1
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 1
M1 - e15
ER -