E-cadherin expression in the subepithelial nevus cells of the giant congenital nevocellular nevi (GCNN) correlates with their migration ability in vitro

N. Matsuda, K. Katsube, Shuji Mikami, Y. Katsuki, H. Iseki, M. Mukai, A. Yamaguchi, Y. Takano, T. Nakajima, H. Nakajima, Kazuo Kishi

Research output: Contribution to journalArticle

Abstract

Background: Giant congenital nevocellular nevi (GCNN) are histologically characterized by the broad distribution of nevus cells in the epidermis and dermis. Objective: To characterize E-cadherin in GCNN and define its role in nevic cell migrations. Methods: Twenty-four cases were immunohistochemically examined and in five cases cells were isolated for primary culture for migration assays. Results: The nevus cells in the superficial region showed the immunoreactivity of E-cadherin in a membranous pattern, but those in the deep part of dermis had little immunoreactivity. Ultra-structural analysis of the superficial nevus cells revealed that E-cadherin immunodeposits in the fibrillar processes around the cell body in a spotted pattern. This distribution pattern is quite different from that in the adherens junction of skin squamous epithelial cells. Boyden chamber experiments were performed using primary cultures of intradermal nevus cells. EDTA pretreatment reduced cell migration to the E-cadherin positive side when the E-cadherin positive population was relatively large in the primary cultures. Conclusions: These results indicate that E-cadherin in the nevus cells may affect nevus cell motility rather than intercellular attachment.

Original languageEnglish
Pages (from-to)21-30
Number of pages10
JournalJournal of Dermatological Science
Volume52
Issue number1
DOIs
Publication statusPublished - 2008 Oct

Fingerprint

Nevus
Giant Cells
Cadherins
Cell Movement
Dermis
Intradermal Nevus
Adherens Junctions
Structural analysis
Edetic Acid
In Vitro Techniques
Assays
Epidermis
Skin
Cells
Epithelial Cells
Population
Experiments

Keywords

  • β-catenin
  • Boyden chamber
  • E-cadherin
  • Migration
  • Nevus
  • Pigment cells

ASJC Scopus subject areas

  • Dermatology

Cite this

E-cadherin expression in the subepithelial nevus cells of the giant congenital nevocellular nevi (GCNN) correlates with their migration ability in vitro. / Matsuda, N.; Katsube, K.; Mikami, Shuji; Katsuki, Y.; Iseki, H.; Mukai, M.; Yamaguchi, A.; Takano, Y.; Nakajima, T.; Nakajima, H.; Kishi, Kazuo.

In: Journal of Dermatological Science, Vol. 52, No. 1, 10.2008, p. 21-30.

Research output: Contribution to journalArticle

Matsuda, N. ; Katsube, K. ; Mikami, Shuji ; Katsuki, Y. ; Iseki, H. ; Mukai, M. ; Yamaguchi, A. ; Takano, Y. ; Nakajima, T. ; Nakajima, H. ; Kishi, Kazuo. / E-cadherin expression in the subepithelial nevus cells of the giant congenital nevocellular nevi (GCNN) correlates with their migration ability in vitro. In: Journal of Dermatological Science. 2008 ; Vol. 52, No. 1. pp. 21-30.
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AU - Matsuda, N.

AU - Katsube, K.

AU - Mikami, Shuji

AU - Katsuki, Y.

AU - Iseki, H.

AU - Mukai, M.

AU - Yamaguchi, A.

AU - Takano, Y.

AU - Nakajima, T.

AU - Nakajima, H.

AU - Kishi, Kazuo

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AB - Background: Giant congenital nevocellular nevi (GCNN) are histologically characterized by the broad distribution of nevus cells in the epidermis and dermis. Objective: To characterize E-cadherin in GCNN and define its role in nevic cell migrations. Methods: Twenty-four cases were immunohistochemically examined and in five cases cells were isolated for primary culture for migration assays. Results: The nevus cells in the superficial region showed the immunoreactivity of E-cadherin in a membranous pattern, but those in the deep part of dermis had little immunoreactivity. Ultra-structural analysis of the superficial nevus cells revealed that E-cadherin immunodeposits in the fibrillar processes around the cell body in a spotted pattern. This distribution pattern is quite different from that in the adherens junction of skin squamous epithelial cells. Boyden chamber experiments were performed using primary cultures of intradermal nevus cells. EDTA pretreatment reduced cell migration to the E-cadherin positive side when the E-cadherin positive population was relatively large in the primary cultures. Conclusions: These results indicate that E-cadherin in the nevus cells may affect nevus cell motility rather than intercellular attachment.

KW - β-catenin

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KW - Migration

KW - Nevus

KW - Pigment cells

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