E-cadherin gene mutations in signet ring cell carcinoma of the stomach

Hiromi Muta, Masayuki Noguchi, Yae Kanai, Atsushi Ochiai, Hajime Nawata, Setsuo Hirohashi

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

To clarify the significance of E-cadherin gene alterations in the development of diffuse-type adenocarcinoma of the stomach, we analyzed mutations of the E-cadherin gene using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method followed by direct sequencing. Twenty-two signet ring cell carcinomas of the stomach (10 intramucosal and 12 advanced cancers) were examined. Genomic DNA was extracted separately from cancerous and non-cancerous tissues and PCR-SSCP analysis was performed on exons 5 to 9 and the adjacent 30- to 40 base pair intron sequences of the E-cadherin gene. Mobility shifts were found in 2 of the 10 intramucosal cancers. In 2 of the 12 advanced cancers, abnormalities of the E-cadherin gene were observed in intramucosal lesions as well as in deeply invaded areas. These results indicated that E-cadherin gene mutations are an early event in the development of signet ring cell carcinoma of the stomach. Direct sequencing revealed that the locations of mutations of the E-cadherin gene included the branch point sequence in the intron which is responsible for RNA splicing. Reverse transcriptase-PCR demonstrated aberrant RNA splicing in a case with a branch point mutation, suggesting that branch point mutations play an important role in functional modifications of E-cadherin in signet ring cell carcinoma of the stomach.

Original languageEnglish
Pages (from-to)843-848
Number of pages6
JournalJapanese Journal of Cancer Research
Volume87
Issue number8
Publication statusPublished - 1996 Aug
Externally publishedYes

Fingerprint

Signet Ring Cell Carcinoma
Cadherins
Stomach
Mutation
Genes
RNA Splicing
Point Mutation
Introns
Neoplasms
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Base Pairing
Exons
Adenocarcinoma
DNA

Keywords

  • E-cadherin
  • Gene mutation
  • Signet ring cell carcinoma
  • Stomach cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Muta, H., Noguchi, M., Kanai, Y., Ochiai, A., Nawata, H., & Hirohashi, S. (1996). E-cadherin gene mutations in signet ring cell carcinoma of the stomach. Japanese Journal of Cancer Research, 87(8), 843-848.

E-cadherin gene mutations in signet ring cell carcinoma of the stomach. / Muta, Hiromi; Noguchi, Masayuki; Kanai, Yae; Ochiai, Atsushi; Nawata, Hajime; Hirohashi, Setsuo.

In: Japanese Journal of Cancer Research, Vol. 87, No. 8, 08.1996, p. 843-848.

Research output: Contribution to journalArticle

Muta, H, Noguchi, M, Kanai, Y, Ochiai, A, Nawata, H & Hirohashi, S 1996, 'E-cadherin gene mutations in signet ring cell carcinoma of the stomach', Japanese Journal of Cancer Research, vol. 87, no. 8, pp. 843-848.
Muta H, Noguchi M, Kanai Y, Ochiai A, Nawata H, Hirohashi S. E-cadherin gene mutations in signet ring cell carcinoma of the stomach. Japanese Journal of Cancer Research. 1996 Aug;87(8):843-848.
Muta, Hiromi ; Noguchi, Masayuki ; Kanai, Yae ; Ochiai, Atsushi ; Nawata, Hajime ; Hirohashi, Setsuo. / E-cadherin gene mutations in signet ring cell carcinoma of the stomach. In: Japanese Journal of Cancer Research. 1996 ; Vol. 87, No. 8. pp. 843-848.
@article{abd489a49224450f8f437e3505a94f19,
title = "E-cadherin gene mutations in signet ring cell carcinoma of the stomach",
abstract = "To clarify the significance of E-cadherin gene alterations in the development of diffuse-type adenocarcinoma of the stomach, we analyzed mutations of the E-cadherin gene using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method followed by direct sequencing. Twenty-two signet ring cell carcinomas of the stomach (10 intramucosal and 12 advanced cancers) were examined. Genomic DNA was extracted separately from cancerous and non-cancerous tissues and PCR-SSCP analysis was performed on exons 5 to 9 and the adjacent 30- to 40 base pair intron sequences of the E-cadherin gene. Mobility shifts were found in 2 of the 10 intramucosal cancers. In 2 of the 12 advanced cancers, abnormalities of the E-cadherin gene were observed in intramucosal lesions as well as in deeply invaded areas. These results indicated that E-cadherin gene mutations are an early event in the development of signet ring cell carcinoma of the stomach. Direct sequencing revealed that the locations of mutations of the E-cadherin gene included the branch point sequence in the intron which is responsible for RNA splicing. Reverse transcriptase-PCR demonstrated aberrant RNA splicing in a case with a branch point mutation, suggesting that branch point mutations play an important role in functional modifications of E-cadherin in signet ring cell carcinoma of the stomach.",
keywords = "E-cadherin, Gene mutation, Signet ring cell carcinoma, Stomach cancer",
author = "Hiromi Muta and Masayuki Noguchi and Yae Kanai and Atsushi Ochiai and Hajime Nawata and Setsuo Hirohashi",
year = "1996",
month = "8",
language = "English",
volume = "87",
pages = "843--848",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - E-cadherin gene mutations in signet ring cell carcinoma of the stomach

AU - Muta, Hiromi

AU - Noguchi, Masayuki

AU - Kanai, Yae

AU - Ochiai, Atsushi

AU - Nawata, Hajime

AU - Hirohashi, Setsuo

PY - 1996/8

Y1 - 1996/8

N2 - To clarify the significance of E-cadherin gene alterations in the development of diffuse-type adenocarcinoma of the stomach, we analyzed mutations of the E-cadherin gene using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method followed by direct sequencing. Twenty-two signet ring cell carcinomas of the stomach (10 intramucosal and 12 advanced cancers) were examined. Genomic DNA was extracted separately from cancerous and non-cancerous tissues and PCR-SSCP analysis was performed on exons 5 to 9 and the adjacent 30- to 40 base pair intron sequences of the E-cadherin gene. Mobility shifts were found in 2 of the 10 intramucosal cancers. In 2 of the 12 advanced cancers, abnormalities of the E-cadherin gene were observed in intramucosal lesions as well as in deeply invaded areas. These results indicated that E-cadherin gene mutations are an early event in the development of signet ring cell carcinoma of the stomach. Direct sequencing revealed that the locations of mutations of the E-cadherin gene included the branch point sequence in the intron which is responsible for RNA splicing. Reverse transcriptase-PCR demonstrated aberrant RNA splicing in a case with a branch point mutation, suggesting that branch point mutations play an important role in functional modifications of E-cadherin in signet ring cell carcinoma of the stomach.

AB - To clarify the significance of E-cadherin gene alterations in the development of diffuse-type adenocarcinoma of the stomach, we analyzed mutations of the E-cadherin gene using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method followed by direct sequencing. Twenty-two signet ring cell carcinomas of the stomach (10 intramucosal and 12 advanced cancers) were examined. Genomic DNA was extracted separately from cancerous and non-cancerous tissues and PCR-SSCP analysis was performed on exons 5 to 9 and the adjacent 30- to 40 base pair intron sequences of the E-cadherin gene. Mobility shifts were found in 2 of the 10 intramucosal cancers. In 2 of the 12 advanced cancers, abnormalities of the E-cadherin gene were observed in intramucosal lesions as well as in deeply invaded areas. These results indicated that E-cadherin gene mutations are an early event in the development of signet ring cell carcinoma of the stomach. Direct sequencing revealed that the locations of mutations of the E-cadherin gene included the branch point sequence in the intron which is responsible for RNA splicing. Reverse transcriptase-PCR demonstrated aberrant RNA splicing in a case with a branch point mutation, suggesting that branch point mutations play an important role in functional modifications of E-cadherin in signet ring cell carcinoma of the stomach.

KW - E-cadherin

KW - Gene mutation

KW - Signet ring cell carcinoma

KW - Stomach cancer

UR - http://www.scopus.com/inward/record.url?scp=0029790319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029790319&partnerID=8YFLogxK

M3 - Article

C2 - 8797891

AN - SCOPUS:0029790319

VL - 87

SP - 843

EP - 848

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 8

ER -