Abstract
To clarify the significance of E-cadherin gene alterations in the development of diffuse-type adenocarcinoma of the stomach, we analyzed mutations of the E-cadherin gene using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method followed by direct sequencing. Twenty-two signet ring cell carcinomas of the stomach (10 intramucosal and 12 advanced cancers) were examined. Genomic DNA was extracted separately from cancerous and non-cancerous tissues and PCR-SSCP analysis was performed on exons 5 to 9 and the adjacent 30- to 40 base pair intron sequences of the E-cadherin gene. Mobility shifts were found in 2 of the 10 intramucosal cancers. In 2 of the 12 advanced cancers, abnormalities of the E-cadherin gene were observed in intramucosal lesions as well as in deeply invaded areas. These results indicated that E-cadherin gene mutations are an early event in the development of signet ring cell carcinoma of the stomach. Direct sequencing revealed that the locations of mutations of the E-cadherin gene included the branch point sequence in the intron which is responsible for RNA splicing. Reverse transcriptase-PCR demonstrated aberrant RNA splicing in a case with a branch point mutation, suggesting that branch point mutations play an important role in functional modifications of E-cadherin in signet ring cell carcinoma of the stomach.
| Original language | English |
|---|---|
| Pages (from-to) | 843-848 |
| Number of pages | 6 |
| Journal | Japanese Journal of Cancer Research |
| Volume | 87 |
| Issue number | 8 |
| Publication status | Published - 1996 Aug |
| Externally published | Yes |
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Keywords
- E-cadherin
- Gene mutation
- Signet ring cell carcinoma
- Stomach cancer
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
E-cadherin gene mutations in signet ring cell carcinoma of the stomach. / Muta, Hiromi; Noguchi, Masayuki; Kanai, Yae; Ochiai, Atsushi; Nawata, Hajime; Hirohashi, Setsuo.
In: Japanese Journal of Cancer Research, Vol. 87, No. 8, 08.1996, p. 843-848.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - E-cadherin gene mutations in signet ring cell carcinoma of the stomach
AU - Muta, Hiromi
AU - Noguchi, Masayuki
AU - Kanai, Yae
AU - Ochiai, Atsushi
AU - Nawata, Hajime
AU - Hirohashi, Setsuo
PY - 1996/8
Y1 - 1996/8
N2 - To clarify the significance of E-cadherin gene alterations in the development of diffuse-type adenocarcinoma of the stomach, we analyzed mutations of the E-cadherin gene using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method followed by direct sequencing. Twenty-two signet ring cell carcinomas of the stomach (10 intramucosal and 12 advanced cancers) were examined. Genomic DNA was extracted separately from cancerous and non-cancerous tissues and PCR-SSCP analysis was performed on exons 5 to 9 and the adjacent 30- to 40 base pair intron sequences of the E-cadherin gene. Mobility shifts were found in 2 of the 10 intramucosal cancers. In 2 of the 12 advanced cancers, abnormalities of the E-cadherin gene were observed in intramucosal lesions as well as in deeply invaded areas. These results indicated that E-cadherin gene mutations are an early event in the development of signet ring cell carcinoma of the stomach. Direct sequencing revealed that the locations of mutations of the E-cadherin gene included the branch point sequence in the intron which is responsible for RNA splicing. Reverse transcriptase-PCR demonstrated aberrant RNA splicing in a case with a branch point mutation, suggesting that branch point mutations play an important role in functional modifications of E-cadherin in signet ring cell carcinoma of the stomach.
AB - To clarify the significance of E-cadherin gene alterations in the development of diffuse-type adenocarcinoma of the stomach, we analyzed mutations of the E-cadherin gene using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method followed by direct sequencing. Twenty-two signet ring cell carcinomas of the stomach (10 intramucosal and 12 advanced cancers) were examined. Genomic DNA was extracted separately from cancerous and non-cancerous tissues and PCR-SSCP analysis was performed on exons 5 to 9 and the adjacent 30- to 40 base pair intron sequences of the E-cadherin gene. Mobility shifts were found in 2 of the 10 intramucosal cancers. In 2 of the 12 advanced cancers, abnormalities of the E-cadherin gene were observed in intramucosal lesions as well as in deeply invaded areas. These results indicated that E-cadherin gene mutations are an early event in the development of signet ring cell carcinoma of the stomach. Direct sequencing revealed that the locations of mutations of the E-cadherin gene included the branch point sequence in the intron which is responsible for RNA splicing. Reverse transcriptase-PCR demonstrated aberrant RNA splicing in a case with a branch point mutation, suggesting that branch point mutations play an important role in functional modifications of E-cadherin in signet ring cell carcinoma of the stomach.
KW - E-cadherin
KW - Gene mutation
KW - Signet ring cell carcinoma
KW - Stomach cancer
UR - http://www.scopus.com/inward/record.url?scp=0029790319&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029790319&partnerID=8YFLogxK
M3 - Article
C2 - 8797891
AN - SCOPUS:0029790319
VL - 87
SP - 843
EP - 848
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 8
ER -