E-cadherin is involved in the intrahepatic metastasis of hepatocellular carcinoma

Takuya Osada, Michiie Sakamoto, Yoshinori Ino, Akihiro Iwamatsu, Yoshihiro Matsuno, Tetsuichiro Muto, Setsuo Hirohashi

Research output: Contribution to journalArticle

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Abstract

In human hepatocellular carcinoma (HCC), the liver is the major target organ of metastasis, which is known as intrahepatic metastasis. To analyze the mechanism of this metastasis, we established two sublines from the human HCC cell line Li7. Subline Li7HM produced multiple liver metastasis, whereas subline Li7NM never did so after intrasplenic injection into nude mice. Two- dimensional gel electrophoresis and immunoblot analysis showed that only Li7NM expressed vimentin and lacked E-cadherin expression, indicating that this clone had undergone epithelial-mesenchymal transition. We then transfected mouse E-cadherin complementary DNA into Li7NM cells and found that the transfectant cells (EM16.21B.3) formed liver metastasis (8/16 mice) after intrasplenic injection and liver tumors (11/13 mice) after intrahepatic injection, whereas the control cell line formed no tumors. These results suggest that E-cadherin plays an important role in the process of intrahepatic metastasis of HCC.

Original languageEnglish
Pages (from-to)1460-1467
Number of pages8
JournalHepatology
Volume24
Issue number6
DOIs
Publication statusPublished - 1996 Dec
Externally publishedYes

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Cadherins
Hepatocellular Carcinoma
Neoplasm Metastasis
Liver
Injections
Cell Line
Epithelial-Mesenchymal Transition
Electrophoresis, Gel, Two-Dimensional
Vimentin
Nude Mice
Neoplasms
Complementary DNA
Clone Cells

ASJC Scopus subject areas

  • Hepatology

Cite this

E-cadherin is involved in the intrahepatic metastasis of hepatocellular carcinoma. / Osada, Takuya; Sakamoto, Michiie; Ino, Yoshinori; Iwamatsu, Akihiro; Matsuno, Yoshihiro; Muto, Tetsuichiro; Hirohashi, Setsuo.

In: Hepatology, Vol. 24, No. 6, 12.1996, p. 1460-1467.

Research output: Contribution to journalArticle

Osada, T, Sakamoto, M, Ino, Y, Iwamatsu, A, Matsuno, Y, Muto, T & Hirohashi, S 1996, 'E-cadherin is involved in the intrahepatic metastasis of hepatocellular carcinoma', Hepatology, vol. 24, no. 6, pp. 1460-1467. https://doi.org/10.1053/jhep.1996.v24.pm0008938181
Osada, Takuya ; Sakamoto, Michiie ; Ino, Yoshinori ; Iwamatsu, Akihiro ; Matsuno, Yoshihiro ; Muto, Tetsuichiro ; Hirohashi, Setsuo. / E-cadherin is involved in the intrahepatic metastasis of hepatocellular carcinoma. In: Hepatology. 1996 ; Vol. 24, No. 6. pp. 1460-1467.
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AU - Ino, Yoshinori

AU - Iwamatsu, Akihiro

AU - Matsuno, Yoshihiro

AU - Muto, Tetsuichiro

AU - Hirohashi, Setsuo

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AB - In human hepatocellular carcinoma (HCC), the liver is the major target organ of metastasis, which is known as intrahepatic metastasis. To analyze the mechanism of this metastasis, we established two sublines from the human HCC cell line Li7. Subline Li7HM produced multiple liver metastasis, whereas subline Li7NM never did so after intrasplenic injection into nude mice. Two- dimensional gel electrophoresis and immunoblot analysis showed that only Li7NM expressed vimentin and lacked E-cadherin expression, indicating that this clone had undergone epithelial-mesenchymal transition. We then transfected mouse E-cadherin complementary DNA into Li7NM cells and found that the transfectant cells (EM16.21B.3) formed liver metastasis (8/16 mice) after intrasplenic injection and liver tumors (11/13 mice) after intrahepatic injection, whereas the control cell line formed no tumors. These results suggest that E-cadherin plays an important role in the process of intrahepatic metastasis of HCC.

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