E3 ubiquitin ligases SIAH1/2 regulate hypoxiainducible factor-1 (HIF-1)-mediated Th17 cell differentiation

Akiko Matsui-Hasumi, Yayoi Sato, Ayako Uto-Konomi, Satoshi Yamashita, Junji Uehori, Akihiko Yoshimura, Masakatsu Yamashita, Hiroshi Asahara, Shinobu Suzuki, Masato Kubo

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4 Citations (Scopus)

Abstract

IL-17 is known to be a cytokine mainly secreted from Th17 cells, which well associate with autoimmune inflammatory responses. In the generation of Th17 cells, RORc and RORa have pivotal roles in controlling the transcription of Il17. We speculated additional regulation in Il17a transcription and randomly screened a 6344 clone cDNA library to identify specific modulators for Il17a promoter activity. After the screen, the E3 ubiquitin ligases SIAH1 and SIAH2 were investigated further and confirmed to increase Il17a promoter activity in a T-cell line and to promote Th17 development ex vivo. This enhancement was a consequence of enhanced expression of hypoxia-inducible factor- 1a (HIF-1a) protein, which is reported to directly regulate expression of Il17a and Rorgt at the transcriptional level. In the absence of HIF-1a, both ubiquitin ligases had little effect on Th17 cell differentiation. These results suggest that the SIAH1 and SIAH2 play a pivotal role to promote Th17 cell differentiation through maintaining the stability of HIF-1a protein.

Original languageEnglish
Article numberdxx014
Pages (from-to)133-143
Number of pages11
JournalInternational immunology
Volume29
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

Keywords

  • Autoimmune
  • HIF-1
  • Signaling
  • T17

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Matsui-Hasumi, A., Sato, Y., Uto-Konomi, A., Yamashita, S., Uehori, J., Yoshimura, A., Yamashita, M., Asahara, H., Suzuki, S., & Kubo, M. (2017). E3 ubiquitin ligases SIAH1/2 regulate hypoxiainducible factor-1 (HIF-1)-mediated Th17 cell differentiation. International immunology, 29(3), 133-143. [dxx014]. https://doi.org/10.1093/intimm/dxx014