Easy and stable lipid bilayer formation: A droplets-contacting-method in parylene mircopores for multiple ion channel recordings

Yutaro Tsuji, Ryuji Kawano, Toshshihisa Osaka, Hirotaka Sasaki, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes simultaneou us 16 channels ion-channel recordings through membrane proteins reconstituted in bilayer lipid membrannes (BLMs) as shown in Figure 1. Although multiple ion-channel recording is necessary for high-throughput screening (HTS) of drug [1], there are no reports of such systems as far as the authors are aware. We have developed several strategies to construct BLMs arrray for multiple ion-channel recording. The droplets contacting method which forms BLMs at the interface of two contacting droplets of lipid monolayers is one of the simplest and most efficient methods. Since it does not require skilled techniques, it is highly reproducible and can be applied to an automated system. [2]. In this study we particularly used a double well chip (DWC) (Figure 1a [3]) with parylene micro-pores in thedroplets contacting method. The parylene micro-pores confined the BLMs forming areas and subsequently, augmented the mechanical stability [4]. Using the droplets contacting method and parylene micro-pores, we succeeded in the multiple recording in 14 channels, which is the most multiple recording as far as we know. This method can be applied to fully automated membrane protein reconstitution for HTS system using a spotting robot [5].

Original languageEnglish
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages214-217
Number of pages4
DOIs
Publication statusPublished - 2011
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: 2011 Jun 52011 Jun 9

Other

Other2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
CountryChina
CityBeijing
Period11/6/511/6/9

Fingerprint

Lipid bilayers
Ions
Screening
Throughput
Proteins
Membranes
Mechanical stability
Lipids
Monolayers
Robots

Keywords

  • Droplets contacting method
  • Drug screening
  • Lipid bilayer
  • Membrane proteins
  • Micro total analysis system

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Tsuji, Y., Kawano, R., Osaka, T., Sasaki, H., Miki, N., & Takeuchi, S. (2011). Easy and stable lipid bilayer formation: A droplets-contacting-method in parylene mircopores for multiple ion channel recordings. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 214-217). [5969386] https://doi.org/10.1109/TRANSDUCERS.2011.5969386

Easy and stable lipid bilayer formation : A droplets-contacting-method in parylene mircopores for multiple ion channel recordings. / Tsuji, Yutaro; Kawano, Ryuji; Osaka, Toshshihisa; Sasaki, Hirotaka; Miki, Norihisa; Takeuchi, Shoji.

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 214-217 5969386.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsuji, Y, Kawano, R, Osaka, T, Sasaki, H, Miki, N & Takeuchi, S 2011, Easy and stable lipid bilayer formation: A droplets-contacting-method in parylene mircopores for multiple ion channel recordings. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969386, pp. 214-217, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 11/6/5. https://doi.org/10.1109/TRANSDUCERS.2011.5969386
Tsuji Y, Kawano R, Osaka T, Sasaki H, Miki N, Takeuchi S. Easy and stable lipid bilayer formation: A droplets-contacting-method in parylene mircopores for multiple ion channel recordings. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 214-217. 5969386 https://doi.org/10.1109/TRANSDUCERS.2011.5969386
Tsuji, Yutaro ; Kawano, Ryuji ; Osaka, Toshshihisa ; Sasaki, Hirotaka ; Miki, Norihisa ; Takeuchi, Shoji. / Easy and stable lipid bilayer formation : A droplets-contacting-method in parylene mircopores for multiple ion channel recordings. 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. pp. 214-217
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