ECM-Based Gradient Generator for Tunable Surface Environment by Interstitial Flow

Azusa Shimizu, Wei H. Goh, Rahul B. Karyappa, Michinao Hashiomoto, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present an extracellular matrix(ECM)-based microfluidic device that creates a spatial chemical gradient by interstitial flow. Cells located on surfaces of ECM, such as vascular and skin tissues, are influenced by external factors, including interstitial flows, but few methods have been available to recapitulate such conditions. To address this gap, we developed a microfluidic device that can expose cells cultured on the surface of ECM to a continuous concentration gradient created by the interstitial flow. With a 3D printed water-soluble sacrificial mold, we readily fabricated ECM-based microfluidic devices embedding micromixers and a gradient generator. ECM mimicked the permeability of in vivo environments. Our device will serve as a platform to study cellular phenomena occurring on the surface of ECM, such as vascularization and migration of cancer cells.

Original languageEnglish
Title of host publication33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages122-124
Number of pages3
ISBN (Electronic)9781728135809
DOIs
Publication statusPublished - 2020 Jan
Event33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 - Vancouver, Canada
Duration: 2020 Jan 182020 Jan 22

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2020-January
ISSN (Print)1084-6999

Conference

Conference33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
CountryCanada
CityVancouver
Period20/1/1820/1/22

Keywords

  • 3D printing
  • Gradient surface
  • Hydrogel
  • Interstitial flow
  • Microfluidics
  • Sacrificial molding
  • Tissue engineering

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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