ECM-based microchannel for culturingin vitrovascular tissues with simultaneous perfusion and stretch

Azusa Shimizu, Wei Huang Goh, Shun Itai, Michinao Hashimoto, Shigenori Miura, Hiroaki Onoe

Research output: Contribution to journalArticle

Abstract

We present an extracellular matrix (ECM)-based stretchable microfluidic system for culturingin vitrothree-dimensional (3D) vascular tissues, which mimicsin vivoblood vessels. Human umbilical vein endothelial cells (HUVECs) can be cultured under perfusion and stretch simultaneously with real-time imaging by our proposed system. Our ECM (transglutaminase (TG) cross-linked gelatin)-based microchannel was fabricated by dissolving water-soluble sacrificial polyvinyl alcohol (PVA) molds printed with a 3D printer. Flows in the microchannel were analyzed under perfusion and stretch. We demonstrated simultaneous perfusion and stretch of TG gelatin-based microchannels culturing HUVECs. We suggest that our TG gelatin-based stretchable microfluidic system proves to be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.

Original languageEnglish
Pages (from-to)1917-1927
Number of pages11
JournalLab on a Chip
Volume20
Issue number11
DOIs
Publication statusPublished - 2020 Jun 7

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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