ECM-based microchannel for culturingin vitrovascular tissues with simultaneous perfusion and stretch

Azusa Shimizu; Wei Huang Goh; Shun Itai; Michinao Hashimoto; Shigenori Miura; Hiroaki Onoe

doi:10.1039/d0lc00254b

ECM-based microchannel for culturingin vitrovascular tissues with simultaneous perfusion and stretch

Azusa Shimizu, Wei Huang Goh, Shun Itai, Michinao Hashimoto, Shigenori Miura, Hiroaki Onoe

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

We present an extracellular matrix (ECM)-based stretchable microfluidic system for culturingin vitrothree-dimensional (3D) vascular tissues, which mimicsin vivoblood vessels. Human umbilical vein endothelial cells (HUVECs) can be cultured under perfusion and stretch simultaneously with real-time imaging by our proposed system. Our ECM (transglutaminase (TG) cross-linked gelatin)-based microchannel was fabricated by dissolving water-soluble sacrificial polyvinyl alcohol (PVA) molds printed with a 3D printer. Flows in the microchannel were analyzed under perfusion and stretch. We demonstrated simultaneous perfusion and stretch of TG gelatin-based microchannels culturing HUVECs. We suggest that our TG gelatin-based stretchable microfluidic system proves to be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.

Original language	English
Pages (from-to)	1917-1927
Number of pages	11
Journal	Lab on a Chip
Volume	20
Issue number	11
DOIs	https://doi.org/10.1039/d0lc00254b
Publication status	Published - 2020 Jun 7

ASJC Scopus subject areas

Bioengineering
Biochemistry
Chemistry(all)
Biomedical Engineering

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10.1039/d0lc00254b

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title = "ECM-based microchannel for culturingin vitrovascular tissues with simultaneous perfusion and stretch",

abstract = "We present an extracellular matrix (ECM)-based stretchable microfluidic system for culturingin vitrothree-dimensional (3D) vascular tissues, which mimicsin vivoblood vessels. Human umbilical vein endothelial cells (HUVECs) can be cultured under perfusion and stretch simultaneously with real-time imaging by our proposed system. Our ECM (transglutaminase (TG) cross-linked gelatin)-based microchannel was fabricated by dissolving water-soluble sacrificial polyvinyl alcohol (PVA) molds printed with a 3D printer. Flows in the microchannel were analyzed under perfusion and stretch. We demonstrated simultaneous perfusion and stretch of TG gelatin-based microchannels culturing HUVECs. We suggest that our TG gelatin-based stretchable microfluidic system proves to be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.",

author = "Azusa Shimizu and Goh, {Wei Huang} and Shun Itai and Michinao Hashimoto and Shigenori Miura and Hiroaki Onoe",

note = "Funding Information: This work was partly supported by Grant-in Aid for Scientific Research (B) (19H04440) from Japan Society for the Promotion of Science (JSPS), Japan, and the research grant from Digital Manufacturing and Design Centre (DManD) at Singapore University of Technology and Design (SUTD) (RGDM1620403), Singapore. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2020.",

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doi = "10.1039/d0lc00254b",

language = "English",

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AU - Shimizu, Azusa

AU - Goh, Wei Huang

AU - Itai, Shun

AU - Hashimoto, Michinao

AU - Miura, Shigenori

AU - Onoe, Hiroaki

N1 - Funding Information: This work was partly supported by Grant-in Aid for Scientific Research (B) (19H04440) from Japan Society for the Promotion of Science (JSPS), Japan, and the research grant from Digital Manufacturing and Design Centre (DManD) at Singapore University of Technology and Design (SUTD) (RGDM1620403), Singapore. Publisher Copyright: © The Royal Society of Chemistry 2020.

PY - 2020/6/7

Y1 - 2020/6/7

N2 - We present an extracellular matrix (ECM)-based stretchable microfluidic system for culturingin vitrothree-dimensional (3D) vascular tissues, which mimicsin vivoblood vessels. Human umbilical vein endothelial cells (HUVECs) can be cultured under perfusion and stretch simultaneously with real-time imaging by our proposed system. Our ECM (transglutaminase (TG) cross-linked gelatin)-based microchannel was fabricated by dissolving water-soluble sacrificial polyvinyl alcohol (PVA) molds printed with a 3D printer. Flows in the microchannel were analyzed under perfusion and stretch. We demonstrated simultaneous perfusion and stretch of TG gelatin-based microchannels culturing HUVECs. We suggest that our TG gelatin-based stretchable microfluidic system proves to be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.

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ECM-based microchannel for culturingin vitrovascular tissues with simultaneous perfusion and stretch

Abstract

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