ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture

Azusa Shimizu, Wei Huang Goh, Michinao Hashimoto, Shigenori Miura, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present an extracellular matrix (ECM)-based stretchable microfluidic system for in vitro three-dimensional (3D) tissue culture, which mimics the in vivo blood vessels. The target cells (ex. endothelial cells (ECs)) can be cultured with perfusion and stretch simultaneously in our proposed system. Our ECM (collagen)-based microfluidic device was fabricated by dissolving water-soluble sacrificial molds fabricated with 3D printer. We demonstrated perfusion and stretch of collagen microfluidic devices with culturing human umbilical vein endothelial cells (HUVECs), showing that the cultured cells were stretched along the expansion of the collagen microchannel. We believe that our collagen-based microfluidic stretch system could be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.

Original languageEnglish
Title of host publication2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages752-755
Number of pages4
ISBN (Electronic)9781728120072
DOIs
Publication statusPublished - 2019 Jun 1
Event20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany
Duration: 2019 Jun 232019 Jun 27

Publication series

Name2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
CountryGermany
CityBerlin
Period19/6/2319/6/27

Fingerprint

Tissue Culture
Tissue culture
Collagen
Microfluidics
collagens
Stretch
Endothelial Cells
microfluidic devices
Endothelial cells
matrices
3D printers
Mechanotransduction
Cultured Cells
printers
Blood Vessels
Microchannel
blood vessels
Veins
Blood vessels
Molds

Keywords

  • Collagen
  • Endothelial cell
  • Mechanical stress
  • Microfluidics
  • Tissue engineering

ASJC Scopus subject areas

  • Process Chemistry and Technology
  • Spectroscopy
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Control and Optimization
  • Instrumentation

Cite this

Shimizu, A., Goh, W. H., Hashimoto, M., Miura, S., & Onoe, H. (2019). ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 752-755). [8808795] (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808795

ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. / Shimizu, Azusa; Goh, Wei Huang; Hashimoto, Michinao; Miura, Shigenori; Onoe, Hiroaki.

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 752-755 8808795 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shimizu, A, Goh, WH, Hashimoto, M, Miura, S & Onoe, H 2019, ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808795, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., pp. 752-755, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 19/6/23. https://doi.org/10.1109/TRANSDUCERS.2019.8808795
Shimizu A, Goh WH, Hashimoto M, Miura S, Onoe H. ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 752-755. 8808795. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). https://doi.org/10.1109/TRANSDUCERS.2019.8808795
Shimizu, Azusa ; Goh, Wei Huang ; Hashimoto, Michinao ; Miura, Shigenori ; Onoe, Hiroaki. / ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 752-755 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).
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