ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture

Azusa Shimizu; Wei Huang Goh; Michinao Hashimoto; Shigenori Miura; Hiroaki Onoe

doi:10.1109/TRANSDUCERS.2019.8808795

ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture

Azusa Shimizu, Wei Huang Goh, Michinao Hashimoto, Shigenori Miura, Hiroaki Onoe

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

We present an extracellular matrix (ECM)-based stretchable microfluidic system for in vitro three-dimensional (3D) tissue culture, which mimics the in vivo blood vessels. The target cells (ex. endothelial cells (ECs)) can be cultured with perfusion and stretch simultaneously in our proposed system. Our ECM (collagen)-based microfluidic device was fabricated by dissolving water-soluble sacrificial molds fabricated with 3D printer. We demonstrated perfusion and stretch of collagen microfluidic devices with culturing human umbilical vein endothelial cells (HUVECs), showing that the cultured cells were stretched along the expansion of the collagen microchannel. We believe that our collagen-based microfluidic stretch system could be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.

Original language	English
Title of host publication	2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	752-755
Number of pages	4
ISBN (Electronic)	9781728120072
DOIs	https://doi.org/10.1109/TRANSDUCERS.2019.8808795
Publication status	Published - 2019 Jun
Event	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany Duration: 2019 Jun 23 → 2019 Jun 27

Publication series

Name	2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Country/Territory	Germany
City	Berlin
Period	19/6/23 → 19/6/27

Keywords

Collagen
Endothelial cell
Mechanical stress
Microfluidics
Tissue engineering

ASJC Scopus subject areas

Process Chemistry and Technology
Spectroscopy
Electrical and Electronic Engineering
Mechanical Engineering
Electronic, Optical and Magnetic Materials
Control and Optimization
Instrumentation

Access to Document

10.1109/TRANSDUCERS.2019.8808795

Cite this

Shimizu, A., Goh, W. H., Hashimoto, M., Miura, S., & Onoe, H. (2019). ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 752-755). [8808795] (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808795

ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. / Shimizu, Azusa; Goh, Wei Huang; Hashimoto, Michinao et al.
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 752-755 8808795 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shimizu, A, Goh, WH, Hashimoto, M, Miura, S & Onoe, H 2019, ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808795, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., pp. 752-755, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 19/6/23. https://doi.org/10.1109/TRANSDUCERS.2019.8808795

Shimizu A, Goh WH, Hashimoto M, Miura S, Onoe H. ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 752-755. 8808795. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). doi: 10.1109/TRANSDUCERS.2019.8808795

Shimizu, Azusa ; Goh, Wei Huang ; Hashimoto, Michinao et al. / ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 752-755 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

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abstract = "We present an extracellular matrix (ECM)-based stretchable microfluidic system for in vitro three-dimensional (3D) tissue culture, which mimics the in vivo blood vessels. The target cells (ex. endothelial cells (ECs)) can be cultured with perfusion and stretch simultaneously in our proposed system. Our ECM (collagen)-based microfluidic device was fabricated by dissolving water-soluble sacrificial molds fabricated with 3D printer. We demonstrated perfusion and stretch of collagen microfluidic devices with culturing human umbilical vein endothelial cells (HUVECs), showing that the cultured cells were stretched along the expansion of the collagen microchannel. We believe that our collagen-based microfluidic stretch system could be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.",

keywords = "Collagen, Endothelial cell, Mechanical stress, Microfluidics, Tissue engineering",

author = "Azusa Shimizu and Goh, {Wei Huang} and Michinao Hashimoto and Shigenori Miura and Hiroaki Onoe",

note = "Funding Information: This work was partly supported by Keio University Special Grant-in-Aid for Innovative Collaborative Research Projects from Keio University, Japan. Publisher Copyright: {\textcopyright} 2019 IEEE.; 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII ; Conference date: 23-06-2019 Through 27-06-2019",

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doi = "10.1109/TRANSDUCERS.2019.8808795",

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AU - Goh, Wei Huang

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AU - Miura, Shigenori

AU - Onoe, Hiroaki

N1 - Funding Information: This work was partly supported by Keio University Special Grant-in-Aid for Innovative Collaborative Research Projects from Keio University, Japan. Publisher Copyright: © 2019 IEEE.

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N2 - We present an extracellular matrix (ECM)-based stretchable microfluidic system for in vitro three-dimensional (3D) tissue culture, which mimics the in vivo blood vessels. The target cells (ex. endothelial cells (ECs)) can be cultured with perfusion and stretch simultaneously in our proposed system. Our ECM (collagen)-based microfluidic device was fabricated by dissolving water-soluble sacrificial molds fabricated with 3D printer. We demonstrated perfusion and stretch of collagen microfluidic devices with culturing human umbilical vein endothelial cells (HUVECs), showing that the cultured cells were stretched along the expansion of the collagen microchannel. We believe that our collagen-based microfluidic stretch system could be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.

AB - We present an extracellular matrix (ECM)-based stretchable microfluidic system for in vitro three-dimensional (3D) tissue culture, which mimics the in vivo blood vessels. The target cells (ex. endothelial cells (ECs)) can be cultured with perfusion and stretch simultaneously in our proposed system. Our ECM (collagen)-based microfluidic device was fabricated by dissolving water-soluble sacrificial molds fabricated with 3D printer. We demonstrated perfusion and stretch of collagen microfluidic devices with culturing human umbilical vein endothelial cells (HUVECs), showing that the cultured cells were stretched along the expansion of the collagen microchannel. We believe that our collagen-based microfluidic stretch system could be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.

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KW - Endothelial cell

KW - Mechanical stress

KW - Microfluidics

KW - Tissue engineering

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ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture

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