Effect of a neuron-specific actin-binding protein, drebrin A, on cell-substratum adhesion

Keiro Ikeda, Tomoaki Shirao, Masahiro Toda, Hideo Asada, Shigeo Toya, Keiichi Uyemura

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17 Citations (Scopus)

Abstract

Drebrin A expression was induced in non-neuronal L cells via transfection with a vector containing the cDNA of rat drebrin A. Following treatment with colcemid (5 μg/ml) and cytochalasin D (0.5 μg/ml), most L cells collapsed into round cells, while drebrin expressing cells were resistant to the treatment, keeping their cell shapes. Simultaneously, actin filaments and microtubules were disrupted in both cell lines. By quantitative analysis, in the presence of cytochalasin D, the extent of cell spreading and cell attachment in drebrin expressing cells was significantly higher than that in control cells. These results suggest that drebrin A modulates cell-substratum adhesion.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalNeuroscience Letters
Volume194
Issue number3
DOIs
Publication statusPublished - 1995 Jul 21

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Keywords

  • Actin
  • Cell-substratum adhesion
  • Colcemid
  • Cytochalasin D
  • Drebrin
  • Tubulin

ASJC Scopus subject areas

  • Neuroscience(all)

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