Effect of a photosensitization reaction performed during the first 3 min after exposure of rat myocardial cells to talaporfin sodium in vitro

Marika Doi, Emiyu Ogawa, Tsunenori Arai

Research output: Contribution to journalArticle

Abstract

To better understand the mechanism of photodynamic cardiac ablation, we studied the effects of a photosensitization reaction (PR) performed during the first 3 min after rat myocardial cells were exposed to talaporfin sodium. A 3-mm-square microelectrode array with 64 electrodes was used to continuously measure the action potentials of the myocardial cells. A 30 μg/mL talaporfin sodium solution, a chlorine photosensitizer, was used, along with a 663-nm red diode laser (86 mW/cm2 for up to 600 s). The first trough of the measured action potential waveform corresponding to Na+ dynamics decreased exponentially with increasing PR duration. The average decay time of the exponential function from PR onset was 20.1 s. Marked morphological changes in the myocardial cells was observed after the PR. These results indicated that the behavior of the action potential waveform measured by the microelectrode array might be used as a less invasive method to evaluate the electrophysiological effects of a PR on myocardial cells.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalLasers in Medical Science
DOIs
Publication statusAccepted/In press - 2017 Aug 5

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Keywords

  • Action potential
  • Electrophysiological ablation
  • Myocardial cell
  • Photosensitization reaction
  • Talaporfin sodium

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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