Effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia2reperfusion injury

Atsuhiro Arisue, Naoki Shimojima, Masayuki Tomiya, Takayuki Shimizu, Daisuke Harada, Mitsuo Nakayama, Hirofumi Tomita, Masahiro Shinoda, Minoru Tanabe, Ikuro Maruyama, Masaru Mizuno, Tatsuo Kuroda, Go Wakabayashi, Yasuhide Morikawa

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Abstract

Objectives: The usefulness of omega-3 lipid emulsions has been extensively studied. The objectives of the present study were to examine the effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia-reperfusion injury and the underlying mechanism. Methods: A total of 66 rats were divided into three dietary groups (lipid-free, soybean oil, and fish oil groups). Each animal was administered total parenteral nutrition for 3 days, followed by induction of intestinal ischemia for 100 min. Animals subjected to sham surgery served as the controls. Intestinal tissue and blood were harvested 6 and 12 h after the surgery, then, assessment of the histological damage score, plasma-related parameters, and statistical evaluation were performed. Results: The histological damage score in the intestinal tissues was significantly lower in the fish oil group than in the soybean oil group (P = 0.0121). The late-phase urinary level of 8-hydroxy-2-deoxyguanosine was also significantly lower in the fish oil group as compared with that in the other groups (P = 0.0267). Furthermore, the plasma level of high-mobility group box 1 protein was also significantly lower in the fish oil group as compared with that in the lipid-free group (P = 0.0398). Conclusion: It appeared that intravenous administration of an omega-3 lipid emulsion prior to ischemia-reperfusion injury reduced the oxidative stress and severity of tissue damage. Modification of membrane fatty acids may serve as the mechanism underlying this reduction of tissue damage.

Original languageEnglish
Pages (from-to)913-918
Number of pages6
JournalPediatric Surgery International
Volume28
Issue number9
DOIs
Publication statusPublished - 2012 Sep 1

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Keywords

  • HMGB1
  • Ischemia-reperfusion injury
  • Omega-3
  • Oxidative stress

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery

Cite this

Arisue, A., Shimojima, N., Tomiya, M., Shimizu, T., Harada, D., Nakayama, M., Tomita, H., Shinoda, M., Tanabe, M., Maruyama, I., Mizuno, M., Kuroda, T., Wakabayashi, G., & Morikawa, Y. (2012). Effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia2reperfusion injury. Pediatric Surgery International, 28(9), 913-918. https://doi.org/10.1007/s00383-012-3144-0