Effect of antibiotics on redox transformations of arsenic and diversity of arsenite-oxidizing bacteria in sediment microbial communities

Shigeki Yamamura, Keiji Watanabe, Wataru Suda, Shun Tsuboi, Mirai Watanabe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In the present study, we investigated the effect of antibiotics on microbial arsenate (As(V)) reduction and arsenite (As(III)) oxidation in sediments collected from a small pond and eutrophic lake. The As(V)-reducing activities were less susceptible to chloramphenicol in aerobic conditions than in anaerobic conditions. Aerobic As(V) reduction proceeded in the presence of diverse types of antibiotics, suggesting that As-resistant bacteria are widely antibiotic resistant. In contrast, some antibiotics, e.g., chloramphenicol, strongly inhibited aerobic As(III) oxidation. In addition, bacterial As(III) oxidase genes were scarcely amplified and Proteobacteria-related 16S rRNA genes drastically decreased in chloramphenicol-amended cultures. Erythromycin and lincomycin, which successfully target many Gram-positive bacteria, scarcely affected As(III) oxidation, although they decreased the diversity of As(III) oxidase genes. These results indicate that the aerobic As(III) oxidizers in the sediment cultures are mainly composed of Proteobacteria and are more sensitive to certain types of antibiotics than the aerobic As(V) reducers. Our results suggest that antibiotic disturbance of environmental microbial communities may affect the biogeochemical cycle of As.

Original languageEnglish
Pages (from-to)350-357
Number of pages8
JournalEnvironmental Science and Technology
Volume48
Issue number1
DOIs
Publication statusPublished - 2014 Jan 7
Externally publishedYes

Fingerprint

arsenite
Arsenic
antibiotics
microbial community
arsenic
Bacteria
Sediments
Anti-Bacterial Agents
bacterium
Chloramphenicol
sediment
Genes
oxidation
Oxidation
gene
Oxidoreductases
Lincomycin
biogeochemical cycle
Ponds
Erythromycin

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Effect of antibiotics on redox transformations of arsenic and diversity of arsenite-oxidizing bacteria in sediment microbial communities. / Yamamura, Shigeki; Watanabe, Keiji; Suda, Wataru; Tsuboi, Shun; Watanabe, Mirai.

In: Environmental Science and Technology, Vol. 48, No. 1, 07.01.2014, p. 350-357.

Research output: Contribution to journalArticle

Yamamura, Shigeki ; Watanabe, Keiji ; Suda, Wataru ; Tsuboi, Shun ; Watanabe, Mirai. / Effect of antibiotics on redox transformations of arsenic and diversity of arsenite-oxidizing bacteria in sediment microbial communities. In: Environmental Science and Technology. 2014 ; Vol. 48, No. 1. pp. 350-357.
@article{dd3b235654c0435796456c237b3640c9,
title = "Effect of antibiotics on redox transformations of arsenic and diversity of arsenite-oxidizing bacteria in sediment microbial communities",
abstract = "In the present study, we investigated the effect of antibiotics on microbial arsenate (As(V)) reduction and arsenite (As(III)) oxidation in sediments collected from a small pond and eutrophic lake. The As(V)-reducing activities were less susceptible to chloramphenicol in aerobic conditions than in anaerobic conditions. Aerobic As(V) reduction proceeded in the presence of diverse types of antibiotics, suggesting that As-resistant bacteria are widely antibiotic resistant. In contrast, some antibiotics, e.g., chloramphenicol, strongly inhibited aerobic As(III) oxidation. In addition, bacterial As(III) oxidase genes were scarcely amplified and Proteobacteria-related 16S rRNA genes drastically decreased in chloramphenicol-amended cultures. Erythromycin and lincomycin, which successfully target many Gram-positive bacteria, scarcely affected As(III) oxidation, although they decreased the diversity of As(III) oxidase genes. These results indicate that the aerobic As(III) oxidizers in the sediment cultures are mainly composed of Proteobacteria and are more sensitive to certain types of antibiotics than the aerobic As(V) reducers. Our results suggest that antibiotic disturbance of environmental microbial communities may affect the biogeochemical cycle of As.",
author = "Shigeki Yamamura and Keiji Watanabe and Wataru Suda and Shun Tsuboi and Mirai Watanabe",
year = "2014",
month = "1",
day = "7",
doi = "10.1021/es403971s",
language = "English",
volume = "48",
pages = "350--357",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "1",

}

TY - JOUR

T1 - Effect of antibiotics on redox transformations of arsenic and diversity of arsenite-oxidizing bacteria in sediment microbial communities

AU - Yamamura, Shigeki

AU - Watanabe, Keiji

AU - Suda, Wataru

AU - Tsuboi, Shun

AU - Watanabe, Mirai

PY - 2014/1/7

Y1 - 2014/1/7

N2 - In the present study, we investigated the effect of antibiotics on microbial arsenate (As(V)) reduction and arsenite (As(III)) oxidation in sediments collected from a small pond and eutrophic lake. The As(V)-reducing activities were less susceptible to chloramphenicol in aerobic conditions than in anaerobic conditions. Aerobic As(V) reduction proceeded in the presence of diverse types of antibiotics, suggesting that As-resistant bacteria are widely antibiotic resistant. In contrast, some antibiotics, e.g., chloramphenicol, strongly inhibited aerobic As(III) oxidation. In addition, bacterial As(III) oxidase genes were scarcely amplified and Proteobacteria-related 16S rRNA genes drastically decreased in chloramphenicol-amended cultures. Erythromycin and lincomycin, which successfully target many Gram-positive bacteria, scarcely affected As(III) oxidation, although they decreased the diversity of As(III) oxidase genes. These results indicate that the aerobic As(III) oxidizers in the sediment cultures are mainly composed of Proteobacteria and are more sensitive to certain types of antibiotics than the aerobic As(V) reducers. Our results suggest that antibiotic disturbance of environmental microbial communities may affect the biogeochemical cycle of As.

AB - In the present study, we investigated the effect of antibiotics on microbial arsenate (As(V)) reduction and arsenite (As(III)) oxidation in sediments collected from a small pond and eutrophic lake. The As(V)-reducing activities were less susceptible to chloramphenicol in aerobic conditions than in anaerobic conditions. Aerobic As(V) reduction proceeded in the presence of diverse types of antibiotics, suggesting that As-resistant bacteria are widely antibiotic resistant. In contrast, some antibiotics, e.g., chloramphenicol, strongly inhibited aerobic As(III) oxidation. In addition, bacterial As(III) oxidase genes were scarcely amplified and Proteobacteria-related 16S rRNA genes drastically decreased in chloramphenicol-amended cultures. Erythromycin and lincomycin, which successfully target many Gram-positive bacteria, scarcely affected As(III) oxidation, although they decreased the diversity of As(III) oxidase genes. These results indicate that the aerobic As(III) oxidizers in the sediment cultures are mainly composed of Proteobacteria and are more sensitive to certain types of antibiotics than the aerobic As(V) reducers. Our results suggest that antibiotic disturbance of environmental microbial communities may affect the biogeochemical cycle of As.

UR - http://www.scopus.com/inward/record.url?scp=84891766539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891766539&partnerID=8YFLogxK

U2 - 10.1021/es403971s

DO - 10.1021/es403971s

M3 - Article

C2 - 24328206

AN - SCOPUS:84891766539

VL - 48

SP - 350

EP - 357

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 1

ER -