Effect of blood and albumin on pulmonary hypertension and edema in perfused rabbit lungs

S. A. Kraft, Seitaro Fujishima, G. P. McGuire, J. S. Thompson, T. A. Raffin, R. G. Pearl

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Perfusate composition may alter pulmonary hemodynamics and edema formation in perfused lungs. Perfusion for 3 h with Krebs-Henseleit solution with 3% bovine serum albumin did not produce pulmonary hypertension, pulmonary edema (assessed by lung wet-to-dry wt ratio), or increased macromolecular permeability (assessed by 125I-albumin uptake). Addition of blood to hematocrit levels of 10 or 20% resulted in pulmonary hypertension during the final hour of perfusion but not pulmonary edema or increased macromolecular permeability. Pulmonary hypertension during blood perfusion was primarily due to increased precapillary resistance. Perfusion with buffer solution without albumin produced edema and increased macromolecular permeability but not pulmonary hypertension. In lungs perfused with blood (20% hematocrit), thromboxane B2 levels increased in parallel with the pulmonary hypertension, and inhibition of cyclooxygenase or thromboxane synthase with indomethacin or dazmegrel prevented pulmonary hypertension. Perfusion with leukopenic blood (from prior nitrogen mustard administration or from filtration) also prevented pulmonary hypertension. We conclude that blood perfusion produces pulmonary hypertension via thromboxane A2 generation, which depends on leukocyte activation, and that perfusion with buffer solutions without albumin produces edema and increased permeability without pulmonary hypertension.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalJournal of Applied Physiology
Volume78
Issue number2
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Pulmonary Edema
Pulmonary Hypertension
Albumins
Rabbits
Lung
Perfusion
Permeability
Hematocrit
Edema
Buffers
Mechlorethamine
Thromboxane B2
Thromboxane A2
Thromboxanes
Prostaglandin-Endoperoxide Synthases
Bovine Serum Albumin
Indomethacin
Leukocytes
Hemodynamics

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Kraft, S. A., Fujishima, S., McGuire, G. P., Thompson, J. S., Raffin, T. A., & Pearl, R. G. (1995). Effect of blood and albumin on pulmonary hypertension and edema in perfused rabbit lungs. Journal of Applied Physiology, 78(2), 499-504.

Effect of blood and albumin on pulmonary hypertension and edema in perfused rabbit lungs. / Kraft, S. A.; Fujishima, Seitaro; McGuire, G. P.; Thompson, J. S.; Raffin, T. A.; Pearl, R. G.

In: Journal of Applied Physiology, Vol. 78, No. 2, 1995, p. 499-504.

Research output: Contribution to journalArticle

Kraft, SA, Fujishima, S, McGuire, GP, Thompson, JS, Raffin, TA & Pearl, RG 1995, 'Effect of blood and albumin on pulmonary hypertension and edema in perfused rabbit lungs', Journal of Applied Physiology, vol. 78, no. 2, pp. 499-504.
Kraft, S. A. ; Fujishima, Seitaro ; McGuire, G. P. ; Thompson, J. S. ; Raffin, T. A. ; Pearl, R. G. / Effect of blood and albumin on pulmonary hypertension and edema in perfused rabbit lungs. In: Journal of Applied Physiology. 1995 ; Vol. 78, No. 2. pp. 499-504.
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