TY - JOUR
T1 - Effect of Brazilian green propolis on oral pathogens and human periodontal fibroblasts
AU - Oda, Hirotake
AU - Nakagawa, Taneaki
AU - Maruyama, Kosuke
AU - Dono, Yuzuru
AU - Katsuragi, Hiroaki
AU - Sato, Soh
N1 - Funding Information:
This study was supported by The Nippon Dental University School of Life Dentistry at Niigata, Japan and Nihon Natural Therapy Co., Ltd. , Tokyo, Japan. All authors declare no potential conflicts related to this study.
Publisher Copyright:
© 2015 Japanese Association for Oral Biology.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Objectives Plaque biofilm is the main etiological factor in dental caries and periodontitis. This study aimed to evaluate the effects of Brazilian green propolis (BGP), which has antibacterial and anti-inflammatory properties, on the growth of oral pathogens, and its cytotoxicity to adult human gingival fibroblasts (HGFs) and periodontal ligament fibroblasts (HPDLFs). Methods Streptococcus mutans, Streptococcus sanguinis, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans were used to evaluate the antibacterial action of BGP. Media containing different concentrations of BGP were prepared, and their effect on bacterial growth was determined by measuring their optical density. The cytotoxicity of BGP to HGFs and HPDLFs was determined by measuring the proliferation and viability of cells with the Alamar Blue assay. Results No significant growth of P. gingivalis, S. mutans, or S. sanguinis was observed when exposed to concentrations of BGP above 2000 μg/ml, but growth of A. actinomycetemcomitans was not affected at this concentration. Growth of P. gingivalis and S. sanguinis was significantly inhibited when exposed to BGP at a concentration of 100 μg/ml. Growth of S. mutans was significantly inhibited in medium containing BGP at 500 μg/ml. Cellular viability (LD50) of HGFs and HPDLFs was not observed at 2000 μg/ml BGP. Conclusions These results suggest that BGP has antibacterial properties but is not significantly cytotoxic to periodontal fibroblasts.
AB - Objectives Plaque biofilm is the main etiological factor in dental caries and periodontitis. This study aimed to evaluate the effects of Brazilian green propolis (BGP), which has antibacterial and anti-inflammatory properties, on the growth of oral pathogens, and its cytotoxicity to adult human gingival fibroblasts (HGFs) and periodontal ligament fibroblasts (HPDLFs). Methods Streptococcus mutans, Streptococcus sanguinis, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans were used to evaluate the antibacterial action of BGP. Media containing different concentrations of BGP were prepared, and their effect on bacterial growth was determined by measuring their optical density. The cytotoxicity of BGP to HGFs and HPDLFs was determined by measuring the proliferation and viability of cells with the Alamar Blue assay. Results No significant growth of P. gingivalis, S. mutans, or S. sanguinis was observed when exposed to concentrations of BGP above 2000 μg/ml, but growth of A. actinomycetemcomitans was not affected at this concentration. Growth of P. gingivalis and S. sanguinis was significantly inhibited when exposed to BGP at a concentration of 100 μg/ml. Growth of S. mutans was significantly inhibited in medium containing BGP at 500 μg/ml. Cellular viability (LD50) of HGFs and HPDLFs was not observed at 2000 μg/ml BGP. Conclusions These results suggest that BGP has antibacterial properties but is not significantly cytotoxic to periodontal fibroblasts.
KW - Antibacterial property
KW - Brazilian green propolis
KW - Cytotoxicity
KW - Periodontal fibroblast
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U2 - 10.1016/j.job.2015.11.001
DO - 10.1016/j.job.2015.11.001
M3 - Article
AN - SCOPUS:84955572638
SN - 1349-0079
VL - 58
SP - 50
EP - 54
JO - Journal of Oral Biosciences
JF - Journal of Oral Biosciences
IS - 2
ER -