Effect of brief hypoxia on reperfusion arrhythmias and release of Ca2+ by rat heart homogenate blocked by ryanodine

Masato Tani, Yasushi Asakura, Hiroshi Hasegawa, Ken Shinmura, Yoshinori Ebihara, Yoshiro Nakamura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives: We previously reported that a brief period of hypoxic perfusion (BHP) prior to ischemia in rat hearts improved functional recovery upon reperfusion with reduced Ca2+ overload. The present study was designed to determine whether the effect of BHP would be associated with a reduction in reperfusion arrhythmias and a preservation of function of the sarcoplasmic reticulum (SR). Methods: Hearts were subjected to 40 min of global ischemia and 30 min of reperfusion after a 20 min period of oxygenated perfusion (oxygenated group: OG), or a 10 min period of oxygenation and 10 min of hypoxic perfusion (hypoxic group: HG). We evaluated the release of Ca2+ by SR blocked by ryanodine, the recovery of left ventricular function, and the reperfusion induced ventricular tachycardia/fibrillation (VT/VF). Results: Functional recovery improved and the incidence and duration of reperfusion VT/VF were reduced in HG. In HG the uptake of Ca2+ in SR decreased during ischemia, but this decrease was less than that in OG. However, recovery of Ca2+ uptake after reperfusion did not differ between groups. The release of Ca2+ by SR blocked by ryanodine was inhibited in HG throughout the ischemia-reperfusion sequence. Conclusions: Observations suggest that the benefits of BHP on recovery of function and reperfusion arrhythmias were associated with a decrease in release of Ca2+ by SR blocked by ryanodine.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalCardiovascular Research
Volume31
Issue number2
DOIs
Publication statusPublished - 1996 Feb

Fingerprint

Ryanodine
Reperfusion
Cardiac Arrhythmias
Sarcoplasmic Reticulum
Perfusion
Ischemia
Hypoxia
Recovery of Function
Ventricular Fibrillation
Ventricular Tachycardia
Left Ventricular Function
Incidence

Keywords

  • Arrhythmias
  • Contractile function
  • Myocardial hypoxia
  • Rat, heart
  • Reperfusion
  • Ryanodine
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Effect of brief hypoxia on reperfusion arrhythmias and release of Ca2+ by rat heart homogenate blocked by ryanodine. / Tani, Masato; Asakura, Yasushi; Hasegawa, Hiroshi; Shinmura, Ken; Ebihara, Yoshinori; Nakamura, Yoshiro.

In: Cardiovascular Research, Vol. 31, No. 2, 02.1996, p. 263-269.

Research output: Contribution to journalArticle

Tani, Masato ; Asakura, Yasushi ; Hasegawa, Hiroshi ; Shinmura, Ken ; Ebihara, Yoshinori ; Nakamura, Yoshiro. / Effect of brief hypoxia on reperfusion arrhythmias and release of Ca2+ by rat heart homogenate blocked by ryanodine. In: Cardiovascular Research. 1996 ; Vol. 31, No. 2. pp. 263-269.
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